UBA5 Protein (His tag)
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- Target See all UBA5 Proteins
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This UBA5 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product UBA5 Protein
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Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 197-404) protein (His-IF2DI Tag)
UBA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (His tag)UBA5 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (GST tag)UBA5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (AA 1-404) protein (Strep Tag)Crystallography grade UBA5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBA5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Ubiquitin-like modifier-activating enzyme 5, THIFP1, UBE1DC1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 1 MM DTT, 10% glycerol, 50 mM NaCl
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- UBA5 (Ubiquitin-Like Modifier Activating Enzyme 5 (UBA5))
- Alternative Name
- UBA5 (UBA5 Products)
- Synonyms
- cb972 Protein, ube1dc1 Protein, UBE1DC1 Protein, THIFP1 Protein, 5730525G14Rik Protein, AW240750 Protein, Ube1dc1 Protein, UBAL Protein, ubiquitin-like modifier activating enzyme 5 Protein, ubiquitin like modifier activating enzyme 5 Protein, ubiquitin-activating enzyme E1-like protein Protein, ubiquitin like modifier activating enzyme 5 S homeolog Protein, uba5 Protein, UBA5 Protein, LOC100270786 Protein, Uba5 Protein, uba5.S Protein
- Background
- UBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (Ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SUMO2. Recombinant human UBA5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 47.4kDa (428aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_079094
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