MMACHC Protein (His tag)

Catalog No. ABIN1098298

Product Details

Target: MMACHC (Methylmalonic Aciduria (Cobalamin Deficiency) CblC Type, with Homocystinuria (MMACHC))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This MMACHC protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Purification: purified by chromatography

Purity: > 90 % by SDS - PAGE

Application Details

Comment:

Synonyms: Methylmalonic aciduria and homocystinuria type C protein, RP11-291L19.3, cblC

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/ml (determined by Bradford assay)

Buffer: 20 mM Tris-HCl buffer (pH 8.0) containing 0.15 M NaCl, 10% glycerol, 1 MM DTT

Storage: 4 °C

Storage Comment: Avoid repeated freezing and thawing cycles.

Target Details

Target: MMACHC (Methylmalonic Aciduria (Cobalamin Deficiency) CblC Type, with Homocystinuria (MMACHC))

Alternative Name: MMACHC (MMACHC Products)

Synonyms: cblC Protein, RP11-291L19.3 Protein, RGD1310806 Protein, 1810037K07Rik Protein, methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria Protein, methylmalonic aciduria cblC type, with homocystinuria Protein, MMACHC Protein, Mmachc Protein

Background: The exact function of MMACHC is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this protein are associated with methylmalonic aciduria and homocystinuria type cblC. Recombinant human MMACHC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Molecular Weight: 34 kDa (306aa), confirmed by MALDI-TOF

NCBI Accession: NP_056321