ACADSB Protein (His tag)
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- Target See all ACADSB Proteins
- ACADSB (Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ACADSB protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ACADSB Protein
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Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB) (AA 1-432) protein (GST tag)
ACADSB Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB) (AA 34-432) protein (His tag)ACADSB Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB) (AA 34-432) protein (His tag)Crystallography grade ACADSB Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB) (AA 34-432) protein (His tag)Crystallography grade ACADSB Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Comment
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Synonyms: Short/branched chain specific acyl-CoA dehydrogenase, mitochondrial, 2-MEBCAD, ACAD7, SBCAD
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- ACADSB (Acyl-CoA Dehydrogenase, Short/branched Chain (ACADSB))
- Alternative Name
- ACADSB (ACADSB Products)
- Synonyms
- 2-mebcad Protein, acad7 Protein, sbcad Protein, DDBDRAFT_0185291 Protein, DDBDRAFT_0237707 Protein, DDB_0185291 Protein, DDB_0237707 Protein, 2-MEBCAD Protein, ACAD7 Protein, SBCAD Protein, 1300003O09Rik Protein, BB066609 Protein, acyl-CoA dehydrogenase, short/branched chain Protein, acyl-CoA dehydrogenase short/branched chain Protein, acyl-coenzyme A dehydrogenase, short/branched chain Protein, acyl-CoA dehydrogenase Protein, acyl-CoA dehydrogenase, short/branched chain L homeolog Protein, acyl-Coenzyme A dehydrogenase, short/branched chain Protein, ACADSB Protein, acadsb Protein, sce1166 Protein, acadsb.L Protein, Acadsb Protein
- Background
- Short/branched chain specific acyl-CoA dehydrogenase(ACADSB), is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. ACADSB catalyzes the degradation of L-isoleucine and has the highest affinity for (s)-2-methylbutyryl-CoA, isobutyryl-CoA and 2-methylhexanoyl-CoA as substrates. Defects in ACADSB are the cause of short/branched-chain acyl-CoA dehydrogenase deficiency (SBCADD), an autosomal recessive disorder characterized by an increase of 2-methylbutyrylglycine and 2-methylbutyrylcarnitine in blood and urine. Recombinant human ACADSB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 46.4 kDa (424aa)
- NCBI Accession
- NP_001600
- Pathways
- Monocarboxylic Acid Catabolic Process
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