SIAH1 Protein (His tag)
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- Target See all SIAH1 Proteins
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This SIAH1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 95 % by SDS - PAGE
- Top Product
- Discover our top product SIAH1 Protein
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E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 90-282) protein (His tag)
SIAH1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-282) protein (GST tag)SIAH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-110) protein (GST tag)SIAH1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-282) protein (Strep Tag)Crystallography grade SIAH1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (AA 1-282) protein (Strep Tag)Crystallography grade SIAH1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Comment
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Synonyms: E3 ubiquitin-protein ligase SIAH1, SIAH1A
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 40% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
- Alternative Name
- SIAH1 (SIAH1 Products)
- Synonyms
- SIAH Protein, Siah1 Protein, Siah1a Protein, id:ibd2635 Protein, zgc:56026 Protein, SIAH1A Protein, siah Protein, E3 ubiquitin-protein ligase Siah1 Protein, E3 ubiquitin-protein ligase siah-1 Protein, siah E3 ubiquitin protein ligase 1 Protein, siah E3 ubiquitin protein ligase 1 L homeolog Protein, Tsp_03055 Protein, siah-1 Protein, Siah1 Protein, siah1 Protein, SIAH1 Protein, siah1.L Protein
- Background
- E3 ubiquitin-protein ligase SIAH1, also known as SIAH1, is a member of the seven in absentia homolog (SIAH) family. SIAH1 is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. SIAH1 contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. SIAH1 is known to cause indirect degradation of beta-catenin through formation of a complex with Siah-interacting protein (SIP), Skp1 and Ebi. Recombinant human SIAH1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 24.1 kDa (216aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_003022
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