ATG10 Protein (AA 1-220) (His tag)
Quick Overview for ATG10 Protein (AA 1-220) (His tag) (ABIN1098406)
Target
See all ATG10 ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-220
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Purification tag / Conjugate
- This ATG10 protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MGSMEEDEFI GEKTFQRYCA EFIKHSQQIG DSWEWRPSKD CSDGYMCKIH FQIKNGSVMS HLGASTHGQT CLPMEEAFEL PLDDCEVIET AAASEVIKYE YHVLYSCSYQ VPVLYFRASF LDGRPLTLKD IWEGVHECYK MRLLQGPWDT ITQQEHPILG QPFFVLHPCK TNEFMTPVLK NSQKINKNVN YITSWLSIVG PVVGLNLPLS YAKATSQDER NVP
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Purification
- > 95% by SDS-PAGE
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Autophagy Related 10 (ATG10) (AA 1-215) protein (His tag)
custom-made ATG10 Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (His tag)custom-made ATG10 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Autophagy Related 10 (ATG10) (AA 1-220) protein (GST tag)ATG10 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Autophagy Related 10 (ATG10) (AA 1-220) protein (His-IF2DI Tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Autophagy Related 10 (ATG10) (Transcript Variant 3) protein (His tag)ATG10 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Synonyms: Ubiquitin-like-conjugating enzyme ATG10, APG10, APG10L, pp12616
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.1M NaCl, 10 % glycerol,1 mM DTT
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- ATG10 (Autophagy Related 10 (ATG10))
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Alternative Name
- ATG10
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Background
- Ubiquitin-like-conjugating enzyme ATG10, also known as ATG10, is a 220 amino acid protein that localizes to the cytoplasm and plays a role in autophagy, specifically functioning as an E2-like enzyme that provides Atg recognition sites during autophagosome synthesis. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Recombinant human ATG10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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Molecular Weight
- 27.7 kDa (243aa) confirmed by MALDI-TOF
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NCBI Accession
- NP_113670
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Pathways
- ER-Nucleus Signaling, Autophagy
Target
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