ECSIT Protein (ECSIT) (His tag)
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- Target See all ECSIT Proteins
- ECSIT
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This ECSIT protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product ECSIT Protein
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ECSIT (ECSIT) (AA 19-217) protein (His tag)
ECSIT Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
ECSIT (ECSIT) (AA 20-431) protein (GST tag)ECSIT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ECSIT (ECSIT) (AA 1-431) protein (GST tag)ECSIT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
ECSIT (ECSIT) (AA 49-431) protein (His tag)Crystallography grade ECSIT Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
ECSIT (ECSIT) (AA 49-435) protein (His tag)Crystallography grade ECSIT Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
ECSIT (ECSIT) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)ECSIT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial, SITPEC
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, 10% glycerol,1 MM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- ECSIT
- Alternative Name
- ECSIT (ECSIT Products)
- Synonyms
- CG10610 Protein, DmECSIT Protein, Dmel\\CG10610 Protein, dECSIT Protein, ECSIT Protein, Sitpec Protein, SITPEC Protein, CG10610 gene product from transcript CG10610-RA Protein, ECSIT signalling integrator Protein, ecsit Protein, ECSIT signalling integrator S homeolog Protein, ECSIT Protein, CpipJ_CPIJ003637 Protein, Ecsit Protein, ecsit.S Protein
- Background
- ECSIT is a ubiquitously expressed protein that plays an important role as an adaptor protein in the cytosolic signal transduction cascade events triggered by Toll receptor activation. It was initially identified as a cytoplasmic protein interacting specifically with TNF receptor associated factor (TRAF)-6 in the TLR pathway. Knockdown of ECSIT results in severely impaired complex I assembly and disturbed mitochondrial function. Recombinant human ECSIT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 24.6 kDa (222aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_001135937
- Pathways
- TLR Signaling, Toll-Like Receptors Cascades, SARS-CoV-2 Protein Interactome
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