MBD3 Protein (His tag)
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- Target See all MBD3 Proteins
- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MBD3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 85 % by SDS - PAGE
- Top Product
- Discover our top product MBD3 Protein
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Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-291) protein (GST tag)
MBD3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 70-179) protein (GST tag)MBD3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-285) protein (Strep Tag)Crystallography grade MBD3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Methyl-CpG Binding Domain Protein 3 (MBD3) (AA 1-291) protein (Strep Tag)Crystallography grade MBD3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
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- Comment
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Synonyms: Methyl-CpG-binding domain protein 3, Methyl CpG binding domain protein 3, Methyl-CpG-binding protein MBD3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol,1 MM DTT, 1 MM EDTA
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MBD3 (Methyl-CpG Binding Domain Protein 3 (MBD3))
- Alternative Name
- MBD3 (MBD3 Products)
- Synonyms
- xmbd3 Protein, MGC69548 Protein, MBD3 Protein, DKFZp459N1635 Protein, AI181826 Protein, AU019209 Protein, methyl-CpG binding domain protein 3 S homeolog Protein, methyl-CpG binding domain protein 3 Protein, mbd3.S Protein, mbd3 Protein, MBD3 Protein, Mbd3 Protein
- Background
- Methyl-CpG-binding domain protein 3, also known as MBD3, is a member of the MBD family of transcriptional repressors. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). However, unlike the other family members, MBD3 is not capable of binding to methylated DNA. The predicted MBD3 protein shares 71% and 94% identity with MBD2 (isoform 1) and mouse Mbd3. MBD3 is a subunit of the NuRD, a multisubunit complex containing nucleosome remodeling and histone deacetylase activities. MBD3 mediates the association of metastasis-associated protein 2 (MTA2) with the core histone deacetylase complex. Recombinant human MBD3 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 35.2 kDa (314aa) confirmed by MALDI-TOF
- NCBI Accession
- NP_003917
- Pathways
- Chromatin Binding
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