JOSD1 Protein (His tag)
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- Target See all JOSD1 Proteins
- JOSD1 (Josephin Domain Containing 1 (JOSD1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This JOSD1 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product JOSD1 Protein
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Josephin Domain Containing 1 (JOSD1) (AA 1-202) protein (His tag)
JOSD1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Josephin Domain Containing 1 (JOSD1) (AA 1-202) protein (GST tag)JOSD1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Josephin Domain Containing 1 (JOSD1) (AA 1-202) protein (Strep Tag)Crystallography grade JOSD1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Josephin Domain Containing 1 (JOSD1) (AA 1-202) protein (Strep Tag)Crystallography grade JOSD1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Josephin Domain Containing 1 (JOSD1) (AA 1-202), (full length) protein (His-SUMO Tag)JOSD1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Josephin Domain Containing 1 (JOSD1) protein (Myc-DYKDDDDK Tag)JOSD1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Josephin-1, dJ508I15.2
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 40% glycerol, 5 MM DTT, 2 mM EDTA
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- JOSD1 (Josephin Domain Containing 1 (JOSD1))
- Alternative Name
- JOSD1 (JOSD1 Products)
- Synonyms
- MGC130880 Protein, zgc:153682 Protein, Josephin-1 Protein, DKFZp459E205 Protein, dJ508I15.2 Protein, 1300006C06Rik Protein, mKIAA0063 Protein, RGD1305388 Protein, Josephin domain containing 1 Protein, Josephin domain containing 1 L homeolog Protein, JOSD1 Protein, josd1.L Protein, josd1 Protein, Josd1 Protein
- Background
- JOSD1, also as known as Josephin-1, has low protease activity towards poly-ubiquitin chains (in vitro). JOSD1 is act as a deubiquitinating enzyme. Recently identified ubiquitin-binding sites in the Josephin domain contribute to ubiquitin chain binding and cleavage. Recombinant human JOSD1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 25.6kDa (225aa), confirmed by MALDI-TOF
- NCBI Accession
- NP_055691
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