PYCRL Protein (His tag)
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- Target See all PYCRL Proteins
- PYCRL (Pyrroline-5-Carboxylate Reductase 3 (PYCRL))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This PYCRL protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purification
- purified by chromatography
- Purity
- > 90 % by SDS - PAGE
- Top Product
- Discover our top product PYCRL Protein
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Pyrroline-5-Carboxylate Reductase 3 (PYCRL) (AA 1-274) protein (His tag)
PYCRL Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Pyrroline-5-Carboxylate Reductase 3 (PYCRL) (AA 1-274) protein (GST tag)PYCRL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pyrroline-5-Carboxylate Reductase 3 (PYCRL) (AA 32-131) protein (GST tag)PYCRL Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Pyrroline-5-Carboxylate Reductase 3 (PYCRL) (AA 2-274) protein (His tag)Crystallography grade PYCRL Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrroline-5-Carboxylate Reductase 3 (PYCRL) (AA 2-274) protein (His tag)Crystallography grade PYCRL Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
Pyrroline-5-Carboxylate Reductase 3 (PYCRL) protein (Myc-DYKDDDDK Tag)PYCRL Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3, pyrroline-5-carboxylate reductase 3
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 0.2 M NaCl, 50% glycerol, 2 mM DTT
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- PYCRL (Pyrroline-5-Carboxylate Reductase 3 (PYCRL))
- Alternative Name
- PYCRL (PYCRL Products)
- Synonyms
- 1110058B13Rik Protein, 2700073G24Rik Protein, si:dkey-235d18.3 Protein, pyrroline-5-carboxylate reductase 3 L homeolog Protein, pyrroline-5-carboxylate reductase 3 Protein, pyrroline-5-carboxylate reductase-like Protein, pycr3.L Protein, PYCR3 Protein, Pycrl Protein, Pycr3 Protein, pycr3 Protein
- Background
- PYCRL belongs to the pyrroline-5-carboxylate reductase family and functions as a homodecamer. It may play a critical role in proline bio-synthesis. Proline functions as a non-enzymatic antioxidant to minimize damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the last step of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline using NAD(P)H as the cofactor. Recombinant human PYCRL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
- Molecular Weight
- 31 kDa (297aa), confirmed by MALDI-TOF
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