MIS12 Protein (His tag)
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- Target See all MIS12 Proteins
- MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purification tag / Conjugate
- This MIS12 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS)
- Purity
- > 90 % by SDS - PAGE
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MIS12, MIND Kinetochore Complex Component, Homolog (MIS12) (AA 1-205) protein (GST tag)
MIS12 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
MIS12, MIND Kinetochore Complex Component, Homolog (MIS12) (AA 1-205) protein (Strep Tag)Crystallography grade MIS12 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
MIS12, MIND Kinetochore Complex Component, Homolog (MIS12) (AA 1-206) protein (Strep Tag)Crystallography grade MIS12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
MIS12, MIND Kinetochore Complex Component, Homolog (MIS12) (AA 1-205), (full length) protein (His-SUMO Tag)MIS12 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
MIS12, MIND Kinetochore Complex Component, Homolog (MIS12) protein (Myc-DYKDDDDK Tag)MIS12 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Comment
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Synonyms: Protein MIS12 homolog, hMis12, KNTC2AP, MTW1
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/ml (determined by Bradford assay)
- Buffer
- 20 mM Tris-HCl buffer (pH 8.0) containing 2 M Urea, 10% glycerol
- Storage
- 4 °C
- Storage Comment
- Avoid repeated freezing and thawing cycles.
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- Target
- MIS12 (MIS12, MIND Kinetochore Complex Component, Homolog (MIS12))
- Alternative Name
- MIS12 (MIS12 Products)
- Synonyms
- 2510025F08Rik Protein, KNTC2AP Protein, MTW1 Protein, hMis12 Protein, BcDNA:RE19545 Protein, CG18156 Protein, Dmel\\CG18156 Protein, Mis-12 Protein, dmMis12 Protein, AU044651 Protein, Misc12 Protein, RP23-83I13.9 Protein, mtw1 Protein, kntc2ap Protein, MGC80789 Protein, ARABIDOPSIS MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE Protein, ATMIS12 Protein, MINICHROMOSOME INSTABILITY 12 (MIS12)-LIKE Protein, MINICHROMOSOME INSTABILITY 12 HOMOLOGUE Protein, MIS12 HOMOLOGUE Protein, MOK9.12 Protein, MOK9_12 Protein, MIS12, kinetochore complex component Protein, CG18156 gene product from transcript CG18156-RA Protein, MIS12 kinetochore complex component Protein, MIS12 kinetochore complex component L homeolog Protein, MIS12, MIND kinetochore complex component, homolog (S. pombe) Protein, minichromosome instability 12 (mis12)-like protein Protein, kinetochore protein Mis12 Protein, MIS12 Protein, Mis12 Protein, mis12.L Protein, mis12 Protein
- Background
- Protein MIS12 homolog, also known as MIS12, is a component of the MIS12 complex, which is required for kinetochore formation during mitosis and normal chromosome alignment and segregation. The MIS12 complex consists of MIS12, DSN1, NSL1 and PMF-1. MIS12 is part of a network of complexes that provide microtubule attachment and generates pulling forces from depolymerization. Recombinant human MIS12 protein, fused to His-tag at N-terminus, was expressed in E.coli.
- Molecular Weight
- 26.5kDa (228aa)
- NCBI Accession
- NP_076944
- Pathways
- M Phase
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