MAFK Protein (AA 1-156) (His tag)
Quick Overview for MAFK Protein (AA 1-156) (His tag) (ABIN1098756)
Target
See all MAFK ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 1-156
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Purification tag / Conjugate
- This MAFK protein is labelled with His tag.
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Sequence
- MGSSHHHHHH SSGLVPRGSH MTTNPKPNKA LKVKKEAGEN APVLSDDELV SMSVRELNQH LRGLTKEEVT RLKQRRRTLK NRGYAASCRI KRVTQKEELE RQRVELQQEV EKLARENSSM RLELDALRSK YEALQTFART VARGPVAPSK VATTSVITIV KSTELSSTSV PFSAAS
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Purification
- > 85% by SDS-PAGE
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Transcription Factor MafK (MAFK) (AA 1-156) protein (His tag)
custom-made MAFK Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transcription Factor MafK (MAFK) (AA 1-156) protein (His tag)custom-made MAFK Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Transcription Factor MafK (MAFK) (AA 1-110) protein (GST tag)MAFK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Transcription Factor MafK (MAFK) proteinMAFK Origin: Human Host: Escherichia coli (E. coli) Recombinant SDS
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Synonyms: Transcription factor MafK, NFE2U, P18
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.5 mg/mL
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Buffer
- 20 mM Tris-HCl buffer ( pH 8.0) containing 0.4M urea, 10 % glycerol
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Storage
- 4 °C,-20 °C,-80 °C
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Storage Comment
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- MAFK (Transcription Factor MafK (MAFK))
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Alternative Name
- MafK
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Background
- MAFK belongs to the bZIP family and Maf subfamily. Members of the Maf family of basic region/leucine zipper (bZIP) transcription factors affect transcription in either a positive or negative fashion, depending on their particular protein partner and the context of the target promoter. Small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Recombinant human MAFK protein, fused to His-tag at N-terminus, was expressed in E. coli.
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Molecular Weight
- 19.7 kDa (176aa)
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NCBI Accession
- NP_002351
Target
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