TGFB3 Protein (His tag)

Catalog No. ABIN1112031

Product Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Protein Type: Recombinant

Origin: Human

Source: Tobacco (Nicotiana benthamiana)

Purification tag / Conjugate: This TGFB3 protein is labelled with His tag.

Application: Western Blotting (WB), Immunogen (Imm)

Sequence: HHHHHHALDT NYCFRNLEEN CCVRPLYIDF RQDLGWKWVH EPKGYYANFC SGPCPYLRSA DTTHSTVLGL YNTLNPEASA SPCCVPQDLE PLTILYYVGR TPKVEQLSNM VVKSCKCS

Specificity: Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta3.

Characteristics: Human recombinant protein expressed in Nicotiana benthamiana. Recombinant human TGF beta 3 contains a 6-His tag at the N-terminal end, is produced by transient expression in non-transgenic plants. This product contains no animal-derived components or impurities. Animal free product.
Serological Identification: The protein was electrophoresed under reducing condition on a 15 % SDS-polyacrylamide gel, transferred by electroblotting to a NC membrane and visualized by immune-detection with specific antibody TGF-beta3.
Molecular formula: C600H902N166O174S10.
Isoelectric Point: 6,75.
Extinction coefficient: E 0.1 % (1g/L) = 1.72 (A 280 nm).
This product contains no animal-derived components or impurities. It is produced by transient expression of TGF-beta3 in non-transgenic plants.

Purification: Recombinant human TGF-beta3 contains a 6-His-tag at the N-terminal end and is purified by sequential chromatography (FPLC).

Purity: > 97 % by SDS-PAGE gel

Endotoxin Level: < 0.04 EU/µg protein (LAL method)

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Lyophilized protein should be reconstituted in water following instructions of batch Quality Control sheet. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.

Concentration: 50 ng/µL

Buffer: Tris HCl 0.05 M buffer at pH 7.4.

Handling Advice: Reconstituted protein should be stored in working aliquots at -20 °C and it is recommended to add a carrier protein (0.1 % HSA or BSA). Repeated freezing and thawing is not recommended.

Storage: 4 °C

References

Bocharov, Korzhnev, Blommers, Arvinte, Orekhov, Billeter, Arseniev: "Dynamics-modulated biological activity of transforming growth factor beta3." in: The Journal of biological chemistry, Vol. 277, Issue 48, pp. 46273-9, (2002) (PubMed).

Massagué: "The transforming growth factor-beta family." in: Annual review of cell biology, Vol. 6, pp. 597-641, (1991) (PubMed).

Miller, Pelton, Derynck, Moses: "Transforming growth factor-beta. A family of growth regulatory peptides." in: Annals of the New York Academy of Sciences, Vol. 593, pp. 208-17, (1990) (PubMed).

ten Dijke, Hansen, Iwata, Pieler, Foulkes: "Identification of another member of the transforming growth factor type beta gene family." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, Issue 13, pp. 4715-9, (1988) (PubMed).

Target Details

Target: TGFB3 (Transforming Growth Factor, beta 3 (TGFB3))

Alternative Name: TGF beta 3 (TGFB3 Products)

Synonyms: TGFB3 Protein, ARVD Protein, TGF-beta3 Protein, Tgfb-3 Protein, TGF-B3 Protein, TGFbeta3 Protein, wu:fc78g09 Protein, zgc:92058 Protein, transforming growth factor beta 3 Protein, transforming growth factor, beta 3 Protein, TGFB3 Protein, Tgfb3 Protein, tgfb3 Protein

Background: Synonyms: Transforming growth factor beta-3, TGF-beta-3
Recombinant human TGF-beta3 is a 27.2 kDa protein composed of two identical 118 amino acid peptide chains linked by a single disulphide bond. Transforming growth factor-beta is a family of five related cytokines that have been shown on a wide variety of normal and neoplastic cells, indicating the importance of these homo-dimmer proteins as multi-functional regulators of cellular activity. The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2 and TGF-beta3) signal through the same receptor and elicit similar biological responses. They are involved in physiological processes as embryogenesis, tissue remodelling and wound healing.

Molecular Weight: 27.2 kDa

Gene ID: 7043, 190230

Pathways: Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus