IL12B Protein

Catalog No. ABIN1305060

Product Details

Target: IL12B (Interleukin 12 beta (IL12B))

Protein Type: Recombinant

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA

Brand: BD Pharmingen™

Characteristics: Recombinant mouse IL-12 p40 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered Dulbecco's PBS (pH 7.2) and no preservatives. The recombinant mouse IL-12, p40 is > 95% pure, as determined by SDS-PAGE, and an absorption assay based on the Beer-Lambert law. The recombinant is not tested for endotoxin or sterility.

Purification: The recombinant mouse IL-12, p40 is > 95 % pure, as determined by SDS-PAGE, and an absorption assay based on the Beer-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The recombinant is not tested for endotoxin or sterility.

Application Details

Application Notes: ELISA Standard: The recombinant mouse IL-12 p40 protein is useful as a quantitative standard for measuring mouse IL-12 protein levels in an IL-12 specific sandwich ELISA. For this assay purified anti-mouse IL-12 p40/70, clone C15.6 (Cat. No. 551219) can be used as the capture antibody when paired with biotinlyated anti-mouse IL-12 p40/p70, clone C17.8 (Cat No. 554476) for detection. To obtain standard curves, doubling dilutions of mouse IL-12 p40 ranging from ~4,000 to 30 pg/ml are recommended for inclusion in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Mouse IL-12 (p40)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 40 μg/mL

Buffer: Recombinant mouse IL-12 p40 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered Dulbecco's PBS ( pH 7.2) and no preservatives.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For use as an ELISA standard we recommend carrier-protein concentrations of 5 -10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein** such as human or bovine albumin, aliquoted and stored at -80°C. For use as an ELISA standard we recommend carrier-protein concentrations of 5 -10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

References

Ling, Gately, Gubler, Stern, Lin, Hollfelder, Su, Pan, Hakimi: "Human IL-12 p40 homodimer binds to the IL-12 receptor but does not mediate biologic activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 1, pp. 116-27, (1995) (PubMed).

DAndrea, Aste-Amezaga, Valiante, Ma, Kubin, Trinchieri: "Interleukin 10 (IL-10) inhibits human lymphocyte interferon gamma-production by suppressing natural killer cell stimulatory factor/IL-12 synthesis in accessory cells." in: The Journal of experimental medicine, Vol. 178, Issue 3, pp. 1041-8, (1993) (PubMed).

DAndrea, Rengaraju, Valiante, Chehimi, Kubin, Aste, Chan, Kobayashi, Young, Nickbarg: "Production of natural killer cell stimulatory factor (interleukin 12) by peripheral blood mononuclear cells." in: The Journal of experimental medicine, Vol. 176, Issue 5, pp. 1387-98, (1992) (PubMed).

Schoenhaut, Chua, Wolitzky, Quinn, Dwyer, McComas, Familletti, Gately, Gubler: "Cloning and expression of murine IL-12." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 11, pp. 3433-40, (1992) (PubMed).

Target Details

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12 (p40) (IL12B Products)

Synonyms: CLMF Protein, CLMF2 Protein, IL-12B Protein, NKSF Protein, NKSF2 Protein, p40 Protein, Il-12b Protein, Il12p40 Protein, Il-12p40 Protein, Il12 Protein, IL-12p40 Protein, IL-12 Protein, IL-12 p40 Protein, il12b Protein, il12p40.b Protein, zgc:152789 Protein, IL-12.p40 Protein, IL12p40 Protein, IL12B Protein, CLMF p40 Protein, interleukin 12B Protein, interleukin 12b Protein, interleukin 12Ba Protein, IL12B Protein, Il12b Protein, il12ba Protein

Background: Interleukin 12 (IL-12) is a potent regulator of cell-mediated immune responses. Biologically active IL-12 is secreted by activated B lymphocytes and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD (p35) and 40 kD (p40) subunits. The IL-12 p40 monomer shares amino acid sequence homology with the IL-6 receptor. It has been reported that activated PBMC produce a large excess of IL-12 p40 monomer over the bioactive p70 heterodimer. The IL-12 p40 monomer has been reported to inhibit binding of IL-12 p70 to the IL-12 receptor, but with 20X less effectiveness than the IL-12 p40 homodimer.

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation