IL-10 Protein

Catalog No. ABIN1305073

Product Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity: D36 indicator cells were co-stimulated with recombinant mouse IL-4 (Cat No. 550067) at 50 pg/mL to promote proliferation and were incubated for 24 hours prior to being pulsed with MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide).
Specific Activity: 1.0 - 6.6 x 10^5 ng/mL
ED50: 0.5 - 100 ng/mL

Characteristics: The recombinant mouse IL-10 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution and containing 4 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. Mouse IL-10 was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Recombinant mouse IL-10 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives. Recombinant mouse IL-10 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of mouse IL-10 as measured in chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Mouse IL-10 is useful as a quantitative standard for measuring mouse IL-10 protein levels in an IL-10 specific sandwich ELISA with the purified JES5-2A5 antibody (Cat. No. 551215) as a capture antibody and the biotinylated clone SXC-1 (Cat. No. 554423) or JES-16E3 (Cat. No. 554465) as the detection antibody. To obtain linear standard curves, doubling dilutions of the mouse IL-10 standard from ~2,000 to 15 pg/mL should be included with each ELISA plate. This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems, not for assay of serum or plasma samples. For measuring IL-10 in serum or plasma, the BD OptEIA™ Mouse IL-10 Set (Cat. No. 555252) is specially formulated and recommended.

Comment:

BD Pharmingen™ Recombinant Mouse IL-10 - Reactivity Ms

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of pro-inflammatory T cell mediated immunity. Mouse IL-10 is an 18.8 kD protein containing 160 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Schlaak, Schmitt, Hüls, Meyer zum Büschenfelde, Fleischer: "A sensitive and specific bioassay for the detection of human interleukin-10." in: Journal of immunological methods, Vol. 168, Issue 1, pp. 49-54, (1994) (PubMed).

Howard, OGarra, Ishida, de Waal Malefyt, de Vries: "Biological properties of interleukin 10." in: Journal of clinical immunology, Vol. 12, Issue 4, pp. 239-47, (1992) (PubMed).

Moore, Vieira, Fiorentino, Trounstine, Khan, Mosmann: "Homology of cytokine synthesis inhibitory factor (IL-10) to the Epstein-Barr virus gene BCRFI." in: Science (New York, N.Y.), Vol. 248, Issue 4960, pp. 1230-4, (1990) (PubMed).

Target Details

Target: IL-10 (IL10) (Interleukin 10 (IL10))

Alternative Name: IL-10 (IL10 Products)

Synonyms: CSIF Protein, GVHDS Protein, IL-10 Protein, IL10A Protein, TGIF Protein, Il-10 Protein, IL10X Protein, interleukin 10 Protein, IL10 Protein, Il10 Protein

Background: Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF) shares over 80 % sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the TH2 cell response, which results in elevated antibody production. The net effect of IL-10 action appears to be the inhibition of pro-inflammatory T cell mediated immunity. Mouse IL-10 is an 18.8 kD protein containing 160 amino acid residues.

Pathways: Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location, Cancer Immune Checkpoints