CXCL9 Protein

Catalog No. ABIN1305078

Product Details

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Characteristics: Recombinant human MIG is > 95% pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. Recombinant human MIG is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution containing 10% glycerol and 1mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Purification: Recombinant human MIG is > 95 % pure, as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of human MIG protein, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human MIG is useful as a quantitative standard for determining recombinant human MIG protein levels in an MIG specific sandwich ELISA with the purified B8-11 antibody (Cat. No. 555038) as a capture antibody and the biotinylated B8-6 (Cat. No. 555037) as the detection antibody. To obtain linear standard curves, doubling dilutions of this recombinant human MIG standard from ~2,000 to 15 pg/ml should be included in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human MIG in serum or plasma the BD OptEIA™ Human MIG ELISA Set (Cat. No. 550998) is specially formulated and recommended. Carrier proteins should be pre-screened for possible effects in appropriate experimental system. Carrier proteins may affect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Comment:

BD Pharmingen™ Recombinant Human MIG

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant human MIG is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing 10 % glycerol and 1 mg/mL biotechnology grade, low endotoxin bovine serum albumin, with no preservatives.

Preservative: Azide free

Handling Advice: Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 1 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 -1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 -10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

Storage: -80 °C

Storage Comment: Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Store product at -80°C prior to use or for long term storage of stock solutions. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 1 mg/ml carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 -1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 -10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity.

References

Loetscher, Loetscher, Brass, Meese, Moser: "Lymphocyte-specific chemokine receptor CXCR3: regulation, chemokine binding and gene localization." in: European journal of immunology, Vol. 28, Issue 11, pp. 3696-705, (1998) (PubMed).

Loetscher, Gerber, Loetscher, Jones, Piali, Clark-Lewis, Baggiolini, Moser: "Chemokine receptor specific for IP10 and mig: structure, function, and expression in activated T-lymphocytes." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 963-9, (1997) (PubMed).

Liao, Rabin, Yannelli, Koniaris, Vanguri, Farber: "Human Mig chemokine: biochemical and functional characterization." in: The Journal of experimental medicine, Vol. 182, Issue 5, pp. 1301-14, (1995) (PubMed).

Farber: "HuMig: a new human member of the chemokine family of cytokines." in: Biochemical and biophysical research communications, Vol. 192, Issue 1, pp. 223-30, (1993) (PubMed).

Farber: "A macrophage mRNA selectively induced by gamma-interferon encodes a member of the platelet factor 4 family of cytokines." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, Issue 14, pp. 5238-42, (1990) (PubMed).

Target Details

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: MIG (CXCL9 Products)

Synonyms: CMK Protein, Humig Protein, MIG Protein, SCYB9 Protein, crg-10 Protein, BB139920 Protein, Mig Protein, MuMIG Protein, Scyb9 Protein, C-X-C motif chemokine ligand 9 Protein, chemokine (C-X-C motif) ligand 9 Protein, CXCL9 Protein, Cxcl9 Protein

Background: Monokine induced by gamma interferon (MIG) is a recently described chemokine of the CXC subfamily which is one of the collection of proteins encoded by cytokine responsive genes. MIG is inducible in macrophages, hepatocytes, and endothelial cells. The synthesis of MIG is specifically induced by IFN-γ, but not by IFN-α or bacterial lipopolysaccharides. The full-length secreted protein is predicted to have 103 amino acids and a MW of 11,725 daltons. Recombinant human MIG has been reported to induce transient elevation of [Ca2+]i in human tumor infiltrating T lymphocytes (TIL) and in cultured, activated human peripheral blood-derived lymphocytes, but not in human neutrophils, monocytes, or EBV-transformed B lymphoblastoid cell lines.