IL-7 Protein

Catalog No. ABIN1305105

Product Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Representative Biological Activity: This product has been reported to have biological activity with an ED50 in the range of 0.2 -0.5ng/ml.
Note: Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activities.

Characteristics: The recombinant human IL-7 is > 95% pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law. Endotoxin level is ≤ 0.1 ng per µg of human IL-7, as measured in a chromogenic LAL assay. Recombinant human IL-7 is supplied lyophilized from a solution comprised of 0.22 µm sterile-filtered PBS and containing 50 µg bovine serum albumin per µg of cytokine.

Purification: The recombinant human IL-7 is > 95 % pure as determined by SDS-PAGE and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin level is ≤ 0.1 ng per μg of human IL-7, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: This recombinant human IL-7 is useful as a quantitative standard for measuring human IL-7 protein levels in an IL-7 specific sandwich ELISA with the purified BVD10-40F6 antibody (Cat. No. 554493) as a capture antibody and the biotinylated BVD10-11C10 (Cat. No. 554494) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-7 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Human IL-7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Recombinant human IL-7 is supplied lyophilized from a solution comprised of 0.22 μm sterile-filtered PBS and containing 50 μg bovine serum albumin per μg of cytokine.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to reconstitution, or for long term storage of lyophilized product or reconstituted stock solutions. The product can be reconstituted in sterile neutral buffer containing carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml.
Note: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 25 µg/ml for long term storage.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to reconstitution, or for long term storage of lyophilized product or reconstituted stock solutions. The product can be reconstituted in sterile neutral buffer containing carrier protein such as human or bovine albumin, aliquoted and stored at -80°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. NOTE: Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 25 μg/ml for long term storage.

References

Namen, Williams, Goodwin: "Interleukin-7: a new hematopoietic growth factor." in: Progress in clinical and biological research, Vol. 338, pp. 65-73, (1990) (PubMed).

Goodwin, Lupton, Schmierer, Hjerrild, Jerzy, Clevenger, Gillis, Cosman, Namen: "Human interleukin 7: molecular cloning and growth factor activity on human and murine B-lineage cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 1, pp. 302-6, (1989) (PubMed).

Yokota, Otsuka, Mosmann, Banchereau, DeFrance, Blanchard, De Vries, Lee, Arai: "Isolation and characterization of a human interleukin cDNA clone, homologous to mouse B-cell stimulatory factor 1, that expresses B-cell- and T-cell-stimulating activities." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 16, pp. 5894-8, (1986) (PubMed).

Target Details

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL-7 (IL7 Products)

Synonyms: IL7 Protein, IL-7 Protein, A630026I06Rik Protein, Il-7 Protein, hlb368 Protein, interleukin 7 Protein, IL7 Protein, Il7 Protein

Background: Interleukin-7 (IL-7) is a potent lymphoid cell growth factor which affects pre-B, pro-B, and early T cells. It will also affect mature T cells in combination with other factors, such as IL-2. It is encoded by a protein of 152 amino acid residues. Human IL-7 migrates as a protein of 17 kDa when analyzed by SDS-PAGE.

Pathways: JAK-STAT Signaling