IL-2 Protein

Catalog No. ABIN1305120

Product Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func)

Brand: BD Pharmingen™

Specificity: Biological Activity Activity is tested: in a Proliferation assay using CTLL-2 indicator cells
Specific Activity: 0.6 - 5.0 x 10^8 Units /mg
ED50: 20 - 150 pg/ml
Observed Dose response: 100 fold A Unit is defined as the amount of material required to stimulate a half-maximal response at cytokine saturation.

Characteristics: Recombinant rat IL-2 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution (pH 7.2) and containing 2 mg/ml biotechnology grade, low-endotoxin bovine serum albumin, glycerol and with no preservatives. This recombinant rat IL-2 is a single band of approximately 16.5 kD as determined by SDS-PAGE analysis on 10 - 20% gels run under reducing conditions.

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of rat IL-2 protein, as measured in a LAL chromogenic assay.

Application Details

Application Notes: ELISA Standard Recombinant rat IL-2 is useful as a quantitative standard for measuring rat IL-2 protein levels in an IL-2 specific sandwich ELISA. To obtain linear standard curves, doubling dilutions of this rat IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate.

Comment:

BD Pharmingen™ Recombinant Rat IL-2 - Reactivity Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: Recombinant rat IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution ( pH 7.2) and containing 2 mg/mL biotechnology grade, low-endotoxin bovine serum albumin, glycerol and with no preservatives. This recombinant rat IL-2 is a single band of approximately 16.5 kD as determined by SDS-PAGE analysis on 10 - 20 % gels run under reducing conditions.

Preservative: Azide free

Handling Advice: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Rapidly thaw and quick-spin product prior to use. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein, such as human or bovine albumin, aliquoted and stored at ≤ -70°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 10 µg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

Storage: -80 °C

Storage Comment: This preparation contains no preservatives, thus it should be handled under aseptic conditions. Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. Upon initial thawing the product should be aliquoted into polypropylene microtubes and frozen at -80°C for future use. Alternatively, the product can be diluted in sterile neutral buffer containing not less than 0.5 - 10 mg/ml carrier protein, such as human or bovine albumin, aliquoted and stored at ≤ -70°C. For in vitro biological assay use, we recommend carrier-protein concentrations of 0.5 - 1.0 mg/ml. For use as an ELISA standard we recommend carrier-protein concentrations of 5 - 10 mg/ml. Failure to add carrier protein or store at indicated temperatures may result in a loss of activity. The product should not be diluted to less than 10 μg/ml for long term storage. Carrier proteins should be pre-screened for possible effects in an appropriate experimental system. Carrier proteins may effect experimental results due to toxicity, high endotoxin levels or possible blocking activity.

References

McKnight, Classon: "Biochemical and immunological properties of rat recombinant interleukin-2 and interleukin-4." in: Immunology, Vol. 75, Issue 2, pp. 286-92, (1992) (PubMed).

Thompson, Di Sabato: "Purification and characterization of two forms of rat interleukin-2." in: Cellular immunology, Vol. 114, Issue 1, pp. 12-26, (1988) (PubMed).

Smith: "Interleukin-2: inception, impact, and implications." in: Science (New York, N.Y.), Vol. 240, Issue 4856, pp. 1169-76, (1988) (PubMed).

Yokota, Arai, Lee, Rennick, Mosmann, Arai: "Use of a cDNA expression vector for isolation of mouse interleukin 2 cDNA clones: expression of T-cell growth-factor activity after transfection of monkey cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 1, pp. 68-72, (1985) (PubMed).

Kashima, Nishi-Takaoka, Fujita, Taki, Yamada, Hamuro, Taniguchi: "Unique structure of murine interleukin-2 as deduced from cloned cDNAs." in: Nature, Vol. 313, Issue 6001, pp. 402-4, (1985) (PubMed).

Watson, Mochizuki: "Interleukin 2: a class of T cell growth factors." in: Immunological reviews, Vol. 51, pp. 257-78, (1981) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Synonyms: IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein

Background: Interleukin-2 (IL-2) is a potent, multifunctional cytokine produced by activated T lymphocytes. It is responsible for the activities attributed to T cell growth factor, thymocyte stimulation factor, thymocyte mitogenesis factor, killer helper factor, and T cell replacing factor. IL-2 stimulates the growth and differentiation of T cells, as well as cytokine production by T cells. In addition, IL-2 can contribute to the activation of monocytes/macrophages and NK and LAK cells and can augment B cell growth and differentiation.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation