IL-2 Protein

Catalog No. ABIN1305121

Product Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA, Functional Studies (Func), Intracellular Flow Cytometry (ICFC), Blocking Reagent (BR)

Brand: BD Pharmingen™

Specificity: Biological Activity, The assay range is measured in a proliferation assay using CTLL-2 indicator cells
Specific Activity: 0.06 - 1.0 x 10^9 Units /mg
ED50: 10-150 pg/ml
Observed dose response: 50 fold
Unit is defined as the amount of material required to stimulate a half-maximal response cytokine saturation.

Characteristics: Recombinant human IL-2 is supplied as a frozen liquid comprised of 0.22 µm sterile-filtered aqueous buffered solution, pH 7.2, and containing 2.0 mg/ml biotechnology grade, low endotoxin bovine serum albumin, with no preservatives. The human IL-2 was purified by immunoaffinity hromatography and was found to be > 95% pure by SDS-PAGE analysis, and an absorbance assay based on the Beers-Lambert law.

Purification: Immunoaffinity Chromatography
Recombinant human IL-2 is supplied as a frozen liquid comprised of 0.22 μm sterile-filtered aqueous buffered solution containing glycerol and bovine serum albumin, with no preservatives. Recombinant human IL-2 is ≥ 95 % pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.

Purity: ≥ 95 %

Sterility: 0.22 μm filtered

Endotoxin Level: The endotoxin level is ≤ 0.1 ng per µg of human IL-2, as measured in a chromogenic LAL assay.

Application Details

Application Notes: ELISA Standard: Recombinant human IL-2 is useful as a quantitative standard for measuring human IL-2 protein levels in an IL-2 specific sandwich ELISA with the purified 5344.111 antibody (Cat. No. 555051) as a capture antibody and the biotinylated B33-2 antibody (Cat. No. 555040) as the detection antibody. To obtain linear standard curves, doubling dilutions of this human IL-2 standard from ~2,000 to 15 pg/ml should be included in each ELISA plate. For specific methodology, please visit the protocols section or chapter on ELISA in the Immune Function Handbook, both of which are posted on our web site.
Note: This ELISA pair is recommended primarily for measuring cytokine from experimental cell culture systems. These ELISA reagents are not recommended for assay of serum or plasma samples. For measuring human Il-2 in serum or plasma the Human IL-2 OptEIA™ Set (Cat. No. 555190) and Human IL-2 OptEIA™ Kit II (Cat. No. 550611 ) are specially formulated and recommended. Ligand Blocking Control for Immunofluorescent Staining of Cytokines The recombinant human IL-2 can be used as a blocking control to demonstrate the specificity of IL-2 staining by directly conjugated formats of the MQ1-17H12 anti-human IL-2 antibody. The use of staining controls for the immunofluorescent staining and flow cytometric analysis of cytokine producing cells has been described in detail.

Comment:

BD Pharmingen™ Recombinant Human IL-2 - Reactivity Hu

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 200 μg/mL

Buffer: Frozen aqueous buffered solution containing BSA and glycerol.

Preservative: Azide free

Handling Advice: Interleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. IL-2 exerts its biological effects by binding to specific receptors expressed by various target cells. Human IL-2 is a 15 kD protein containing 133 amino acid residues.

Storage: -80 °C

Storage Comment: Store product at -80°C prior to use or for long term storage of stock solutions. Rapidly thaw and quick-spin product prior to use. Avoid multiple freeze-thaws of product. This preparation contains no preservatives, thus it should be handled under aseptic conditions.

References

Prussin, Metcalfe: "Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies." in: Journal of immunological methods, Vol. 188, Issue 1, pp. 117-28, (1996) (PubMed).

Smith: "Interleukin-2: inception, impact, and implications." in: Science (New York, N.Y.), Vol. 240, Issue 4856, pp. 1169-76, (1988) (PubMed).

Stern, Pan, Urdal, Mochizuki, DeChiara, Blacher, Wideman, Gillis: "Purification to homogeneity and partial characterization of interleukin 2 from a human T-cell leukemia." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, Issue 3, pp. 871-5, (1984) (PubMed).

Taniguchi, Matsui, Fujita, Takaoka, Kashima, Yoshimoto, Hamuro: "Structure and expression of a cloned cDNA for human interleukin-2." in: Nature, Vol. 302, Issue 5906, pp. 305-10, (1983) (PubMed).

Gillis, Ferm, Ou, Smith: "T cell growth factor: parameters of production and a quantitative microassay for activity." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 120, Issue 6, pp. 2027-32, (1978) (PubMed).

Target Details

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL-2 (IL2 Products)

Synonyms: IL2 Protein, IL-2 Protein, il2 Protein, TCGF Protein, lymphokine Protein, Il-2 Protein, POIL2 Protein, interleukin 2 Protein, interleukin-15 Protein, IL2 Protein, il2 Protein, LOC100303688 Protein, Il2 Protein

Background: Interleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. IL-2 exerts its biological effects by binding to specific receptors expressed by various target cells. Human IL-2 is a 15 kD protein containing 133 amino acid residues.

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation