ABCB1 Protein (AA 620-709) (GST tag)
(1 reference)Quick Overview for ABCB1 Protein (AA 620-709) (GST tag) (ABIN1305435)
Target
See all ABCB1 ProteinsProtein Type
Origin
Source
Application
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Protein Characteristics
- AA 620-709
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Purification tag / Conjugate
- This ABCB1 protein is labelled with GST tag.
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Purpose
- ABCB1 (Human) Recombinant Protein (Q01)
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Sequence
- GIYFKLVTMQ TAGNEVELEN AADESKSEID ALEMSSNDSR SSLIRKRSTR RSVRGSQAQD RKLSTKEALD ESIPPVSFWR IMKLNLTEWP
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Characteristics
- Human ABCB1 partial ORF ( NP_000918, 620 a.a. - 709 a.a.) recombinant protein with GST-tag at N-terminal.
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Purification
- in vitro wheat germ expression system
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ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 1-1280) protein (His tag)
custom-made ABCB1 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) (AA 491-694) protein (His-IF2DI Tag)ABCB1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) protein (DYKDDDDK Tag)ABCB1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1) protein (His tag)ABCB1 Origin: Human Host: Insect cells (Sf9) Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. -
Restrictions
- For Research Use only
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Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
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Handling Advice
- Aliquot to avoid repeated freezing and thawing.
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Storage
- -80 °C
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Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Quantitative atlas of membrane transporter proteins: development and application of a highly sensitive simultaneous LC/MS/MS method combined with novel in-silico peptide selection criteria." in: Pharmaceutical research, Vol. 25, Issue 6, pp. 1469-83, (2008) (PubMed).
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: "Quantitative atlas of membrane transporter proteins: development and application of a highly sensitive simultaneous LC/MS/MS method combined with novel in-silico peptide selection criteria." in: Pharmaceutical research, Vol. 25, Issue 6, pp. 1469-83, (2008) (PubMed).
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- ABCB1 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 1 (ABCB1))
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Alternative Name
- ABCB1
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Background
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Full Gene Name: ATP-binding cassette, sub-family B (MDR/TAP), member 1
Synonyms: ABC20,CD243,CLCS,GP170,MDR1,MGC163296,P-GP,PGY1 -
Gene ID
- 5243
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NCBI Accession
- NM_000927
Target
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