HSP27 Protein (AA 1-205) (GST tag)

Catalog No. ABIN1307214

Product Details

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Protein Type: Recombinant

Protein Characteristics: AA 1-205

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This HSP27 protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: HSPB1 (Human) Recombinant Protein (P01)

Sequence: MTERRVPFSL LRGPSWDPFR DWYPHSRLFD QAFGLPRLPE EWSQWLGGSS WPGYVRPLPP AAIESPAVAA PAYSRALSRQ LSSGVSEIRH TADRWRVSLD VNHFAPDELT VKTKDGVVEI TGKHEERQDE HGYISRCFTR KYTLPPGVDP TQVSSSLSPE GTLTVEAPMP KLATQSNEIT IPVTFESRAQ LGGPEAAKSD ETAAK

Characteristics: Human HSPB1 full-length ORF ( NP_001531.1, 1 a.a. - 205 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Imami, Bhavsar, Yu, Brown, Rogers, Finlay, Foster: "Global impact of Salmonella pathogenicity island 2-secreted effectors on the host phosphoproteome." in: Molecular & cellular proteomics : MCP, Vol. 12, Issue 6, pp. 1632-43, (2013) (PubMed).

Iizuka, Okamoto, Matsushita, Kimura, Nagai, Arito, Kurokawa, Masuko, Suematsu, Hirohata, Kato: "Identification of autoantigens specific for systemic lupus erythematosus with central nervous system involvement." in: Lupus, Vol. 19, Issue 6, pp. 717-26, (2010) (PubMed).

Target Details

Target: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name: HSPB1 (HSPB1 Products)

Synonyms: CMT2F Protein, HMN2B Protein, HS.76067 Protein, HSP27 Protein, HSP28 Protein, Hsp25 Protein, SRP27 Protein, 27kDa Protein, Hsp27 Protein, cb153 Protein, cb660 Protein, hsp1 Protein, hsp25 Protein, hsp27 Protein, id:ibd2821 Protein, sb:cb660 Protein, zgc:103437 Protein, HSPB1 Protein, cmt2f Protein, hs.76067 Protein, hsp28 Protein, heat shock protein family B (small) member 1 Protein, heat shock protein 1 Protein, heat shock protein, alpha-crystallin-related, 1 Protein, heat shock protein family B (small) member 1 L homeolog Protein, HSPB1 Protein, Hspb1 Protein, hspb1 Protein, hspb1.L Protein

Background: Full Gene Name: heat shock 27 kDa protein 1
Synonyms: CMT2F,DKFZp586P1322,HMN2B,HS.76067,HSP27,HSP28,Hsp25,SRP27

Gene ID: 3315

NCBI Accession: NM_001540

Pathways: MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling