LEF1 Protein (AA 1-399) (GST tag)
(1 reference)-
- Target See all LEF1 Proteins
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-399
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This LEF1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- LEF1 (Human) Recombinant Protein (P01)
- Sequence
- MPQLSGGGGG GGGDPELCAT DEMIPFKDEG DPQKEKIFAE ISHPEEEGDL ADIKSSLVNE SEIIPASNGH EVARQAQTSQ EPYHDKAREH PDDGKHPDGG LYNKGPSYSS YSGYIMMPNM NNDPYMSNGS LSPPIPRTSN KVPVVQPSHA VHPLTPLITY SDEHFSPGSH PSHIPSDVNS KQGMSRHPPA PDIPTFYPLS PGGVGQITPP LGWQGQPVYP ITGGFRQPYP SSLSVDTSMS RFSHHMIPGP PGPHTTGIPH PAIVTPQVKQ EHPHTDSDLM HVKPQHEQRK EQEPKRPHIK KPLNAFMLYM KEMRANVVAE CTLKESAAIN QILGRRWHAL SREEQAKYYE LARKERQLHM QLYPGWSARD NYGKKKKRKR EKLQESASGT GPRMTAAYI
- Characteristics
- Human LEF1 full-length ORF ( AAH50632, 1 a.a. - 399 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 120-399) protein (His tag)
LEF1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses ELISA, WB
Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 14-123) protein (GST tag)LEF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 33-138) protein (GST tag)LEF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-399) protein (Strep Tag)Crystallography grade LEF1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Lymphoid Enhancer-Binding Factor 1 (LEF1) (AA 1-397) protein (Strep Tag)Crystallography grade LEF1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1." in: Medical molecular morphology, Vol. 46, Issue 1, pp. 14-9, (2013) (PubMed).
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: "Identification of DNA-dependent protein kinase catalytic subunit as a novel interaction partner of lymphocyte enhancer factor 1." in: Medical molecular morphology, Vol. 46, Issue 1, pp. 14-9, (2013) (PubMed).
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- Target
- LEF1 (Lymphoid Enhancer-Binding Factor 1 (LEF1))
- Alternative Name
- LEF1 (LEF1 Products)
- Synonyms
- LEF1 Protein, 3000002B05 Protein, AI451430 Protein, Lef-1 Protein, LEF-1 Protein, TCF10 Protein, TCF1ALPHA Protein, TCF7L3 Protein, lymphoid enhancer binding factor 1 Protein, lymphoid enhancer-binding factor 1 Protein, lymphoid enhancer factor XLEF-1B Protein, LEF1 Protein, lef1 Protein, Lef1 Protein
- Background
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Full Gene Name: lymphoid enhancer-binding factor 1
Synonyms: DKFZp586H0919,TCF1ALPHA - Gene ID
- 51176
- Pathways
- WNT Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Hormone Metabolic Process, Nuclear Hormone Receptor Binding, Chromatin Binding
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