LIG1 Protein (AA 810-919) (GST tag)
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- Target See all LIG1 Proteins
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 810-919
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This LIG1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- LIG1 (Human) Recombinant Protein (Q01)
- Sequence
- QSLKALVLPS PRPYVRIDGA VIPDHWLDPS AVWEVKCADL SLSPIYPAAR GLVDSDKGIS LRFPRFIRVR EDKQPEQATT SAQVACLYRK QSQIQNQQGE DSGSDPEDTY
- Characteristics
- Human LIG1 partial ORF ( NP_000225, 810 a.a. - 919 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919) protein (Strep Tag)
Crystallography grade LIG1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-916) protein (Strep Tag)Crystallography grade LIG1 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Ligase I, DNA, ATP-Dependent (LIG1) protein (Myc-DYKDDDDK Tag)LIG1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919), (full length) protein (GST tag)LIG1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
- Alternative Name
- LIG1 (LIG1 Products)
- Synonyms
- ATLIG1 Protein, DNA ligase 1 Protein, T6D22.23 Protein, ligI Protein, AL033288 Protein, LigI Protein, lig1 Protein, wu:fc54a11 Protein, DNA ligase 1 Protein, DNA ligase 1, putative Protein, ligase I, DNA, ATP-dependent L homeolog Protein, ligase I, DNA, ATP-dependent Protein, LIG1 Protein, PB000674.02.0 Protein, PTRG_06243 Protein, PKH_140260 Protein, lig1.L Protein, Lig1 Protein, lig-1 Protein, lig1 Protein
- Background
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Full Gene Name: ligase I, DNA, ATP-dependent
Synonyms: MGC117397,MGC130025 - Gene ID
- 3978
- NCBI Accession
- NM_000234
- Pathways
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
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