LC3B Protein (AA 1-125) (GST tag)

Catalog No. ABIN1310156

Product Details

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Protein Type: Recombinant

Protein Characteristics: AA 1-125

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This LC3B protein is labelled with GST tag.

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Purpose: MAP1LC3B (Human) Recombinant Protein (P01)

Sequence: MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG MKLSV

Characteristics: Human MAP1LC3B full-length ORF ( AAH18634, 1 a.a. - 125 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Koukourakis, Giatromanolaki, Bottini, Cappelletti, Zanotti, Allevi, Strina, Ardine, Milani, Brugnoli, Martinotti, Ferrero, Bertoni, Ferrozzi, Harris, Generali: "Prospective neoadjuvant analysis of PET imaging and mechanisms of resistance to Trastuzumab shows role of HIF1 and autophagy." in: British journal of cancer, Vol. 110, Issue 9, pp. 2209-16, (2014) (PubMed).

Martinet, Schrijvers, Timmermans, Bult, De Meyer: "Immunohistochemical analysis of macroautophagy: recommendations and limitations." in: Autophagy, Vol. 9, Issue 3, pp. 386-402, (2013) (PubMed).

Koukourakis, Giatromanolaki, Zois, Sivridis: "LC3 immunostaining pitfalls." in: Histopathology, Vol. 62, Issue 6, pp. 962-3, (2013) (PubMed).

Target Details

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3B (MAP1LC3B Products)

Background: Full Gene Name: microtubule-associated protein 1 light chain 3 beta
Synonyms: LC3B,MAP1A/1BLC3

Gene ID: 81631

Pathways: Autophagy