PEPCK Protein (AA 1-640) (GST tag)
(1 reference)-
- Target See all PEPCK Proteins
- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-640
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PEPCK protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PCK2 (Human) Recombinant Protein (P01)
- Sequence
- MAALYRPGLR LNWHGLSPLG WPSCRSIQTL RVLSGDLGQL PTGIRDFVEH SARLCQPEGI HICDGTEAEN TATLTLLEQQ GLIRKLPKYN NCWLARTDPK DVARVESKTV IVTPSQRDTV PLPPGGARGQ LGNWMSPADF QRAVDERFPG CMQGRTMYVL PFSMGPVGSP LSRIGVQLTD SAYVVASMRI MTRLGTPVLQ ALGDGDFVKC LHSVGQPLTG QGEPVSQWPC NPEKTLIGHV PDQREIISFG SGYGGNSLLG KKCFALRIAS RLARDEGWLA EHMLILGITS PAGKKRYVAA AFPSACGKTN LAMMRPALPG WKVECVGDDI AWMRFDSEGR LRAINPENGF FGVAPGTSAT TNPNAMATIQ SNTIFTNVAE TSDGGVYWEG IDQPLPPGVT VTSWLGKPWK PGDKEPCAHP NSRFCAPARQ CPIMDPAWEA PEGVPIDAII FGGRRPKGVP LVYEAFNWRH GVFVGSAMRS ESTAAAEHKG KIIMHDPFAM RPFFGYNFGH YLEHWLSMEG RKGAQLPRIF HVNWFRRDEA GHFLWPGFGE NARVLDWICR RLEGEDSARE TPIGLVPKEG ALDLSGLRAI DTTQLFSLPK DFWEQEVRDI RSYLTEQVNQ DLPKEVLAEL EALERRVHKM
- Characteristics
- Human PCK2 full-length ORF ( AAH01454, 1 a.a. - 640 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) protein (GST tag,His tag)
PEPCK Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 33-640) protein (His tag)Crystallography grade PEPCK Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK) (AA 33-640) protein (His tag)Crystallography grade PEPCK Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Anti-phosphoenolpyruvate carboxykinase 2 antibody in patients with autoimmune hepatitis." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 44, Issue 9, pp. 1019-25, (2014) (PubMed).
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: "Anti-phosphoenolpyruvate carboxykinase 2 antibody in patients with autoimmune hepatitis." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 44, Issue 9, pp. 1019-25, (2014) (PubMed).
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- Target
- PEPCK (phosphoenolpyruvate Carboxykinase 2 (Mitochondrial) (PEPCK))
- Alternative Name
- PCK2 (PEPCK Products)
- Synonyms
- PEPCK Protein, PEPCK-M Protein, PEPCK2 Protein, 1810010O14Rik Protein, 9130022B02Rik Protein, pck Protein, zgc:77867 Protein, id:ibd5157 Protein, pck2 Protein, pck2.S Protein, phosphoenolpyruvate carboxykinase 2, mitochondrial Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) Protein, phosphoenolpyruvate carboxykinase 2 (mitochondrial) L homeolog Protein, PCK2 Protein, Pck2 Protein, pck2 Protein, pck2.L Protein
- Background
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Full Gene Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
Synonyms: PEPCK,PEPCK-M,PEPCK2 - Gene ID
- 5106
- Pathways
- Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis
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