PIAS1 Protein (AA 1-651) (GST tag)

Catalog No. ABIN1314985

Recombinant PIAS1 protein expressed in Wheat germ and has been mentioned in 1 publication.

Quick Overview for PIAS1 Protein (AA 1-651) (GST tag) (ABIN1314985)

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Protein Type: Recombinant

Origin: Human

Source: Wheat germ

Application: Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)

Product Details

Protein Characteristics: AA 1-651

Purification tag / Conjugate: This PIAS1 protein is labelled with GST tag.

Purpose: PIAS1 (Human) Recombinant Protein (P01)

Sequence: MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPATLS PSTIPQLTYD GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK LPFYDLLDEL IKPTSLASDN SQRFRETCFA FALTPQQVQQ ISSSMDISGT KCDFTVQVQL RFCLSETSCP QEDHFPPNLC VKVNTKPCSL PGYLPPTKNG VEPKRPSRPI NITSLVRLST TVPNTIVVSW TAEIGRNYSM AVYLVKQLSS TVLLQRLRAK GIRNPDHSRA LIKEKLTADP DSEIATTSLR VSLLCPLGKM RLTIPCRALT CSHLQCFDAT LYIQMNEKKP TWVCPVCDKK APYEHLIIDG LFMEILKYCT DCDEIQFKED GTWAPMRSKK EVQEVSASYN GVDGCLSSTL EHQVASHHQS SNKNKKVEVI DLTIDSSSDE EEEEPSAKRT CPSLSPTSPL NNKGILSLPH QASPVSRTPS LPAVDTSYIN TSLIQDYRHP FHMTPMPYDL QGLDFFPFLS GDNQHYNTSL LAAAAAAVSD DQDLLHSSRF FPYTSSQMFL DQLSAGGSTS LPTTNGSSSG SNSSLVSSNS LRESHSHTVT NRSSTDTASI FGIIPDIISL D

Characteristics: Human PIAS1 full-length ORF ( NP_057250.1, 1 a.a. - 651 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Chen, Hsu, Ma, Tai, Lee: "CREB SUMOylation by the E3 ligase PIAS1 enhances spatial memory." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 34, Issue 29, pp. 9574-89, (2014) (PubMed).

Target Details

Target: PIAS1 (Protein Inhibitor of Activated STAT, 1 (PIAS1))

Alternative Name: PIAS1

Background: Full Gene Name: protein inhibitor of activated STAT, 1
Synonyms: DDXBP1,GBP,GU/RH-II,MGC141878,MGC141879,ZMIZ3

Gene ID: 8554

NCBI Accession: NM_016166

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C