PPID Protein (AA 1-370) (GST tag)
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- Target See all PPID Proteins
- PPID (Peptidylprolyl Isomerase D (PPID))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-370
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PPID protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PPID (Human) Recombinant Protein (P01)
- Sequence
- MSHPSPQAKP SNPSNPRVFF DVDIGGERVG RIVLELFADI VPKTAENFRA LCTGEKGIGH TTGKPLHFKG CPFHRIIKKF MIQGGDFSNQ NGTGGESIYG EKFEDENFHY KHDREGLLSM ANAGRNTNGS QFFITTVPTP HLDGKHVVFG QVIKGIGVAR ILENVEVKGE KPAKLCVIAE CGELKEGDDG GIFPKDGSGD SHPDFPEDAD IDLKDVDKIL LITEDLKNIG NTFFKSQNWE MAIKKYAEVL RYVDSSKAVI ETADRAKLQP IALSCVLNIG ACKLKMSNWQ GAIDSCLEAL ELDPSNTKAL YRRAQGWQGL KEYDQALADL KKAQGIAPED KAIQAELLKV KQKIKAQKDK EKAVYAKMFA
- Characteristics
- Human PPID full-length ORF ( NP_005029.1, 1 a.a. - 370 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 40184-92, (2011) (PubMed).
: "Complement 1q-binding protein inhibits the mitochondrial permeability transition pore and protects against oxidative stress-induced death." in: The Biochemical journal, Vol. 433, Issue 1, pp. 119-25, (2010) (PubMed).
: "
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Cysteine 203 of cyclophilin D is critical for cyclophilin D activation of the mitochondrial permeability transition pore." in: The Journal of biological chemistry, Vol. 286, Issue 46, pp. 40184-92, (2011) (PubMed).
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- Target
- PPID (Peptidylprolyl Isomerase D (PPID))
- Alternative Name
- PPID (PPID Products)
- Synonyms
- PPID Protein, CYP-40 Protein, CYPD Protein, Cyp-40 Protein, CypD Protein, wu:fb18b07 Protein, zgc:86711 Protein, 4930564J03Rik Protein, Ppidl Protein, Ppif Protein, cyp-40 Protein, cypd Protein, peptidylprolyl isomerase D Protein, peptidylprolyl isomerase D (cyclophilin D) Protein, peptidylprolyl isomerase D (cyclophilin D) S homeolog Protein, PPID Protein, Ppid Protein, ppid Protein, ppid.S Protein
- Background
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Full Gene Name: peptidylprolyl isomerase D
Synonyms: CYP-40,CYPD,MGC33096 - Gene ID
- 5481
- NCBI Accession
- NM_005038
- Pathways
- Nuclear Hormone Receptor Binding
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