PRAME Protein (AA 1-509) (GST tag)
(1 reference)-
- Target See all PRAME Proteins
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-509
- Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PRAME protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PRAME (Human) Recombinant Protein (P01)
- Sequence
- MERRRLRGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR ELFPPLFMAA FDGRHSQTLK AMVQAWPFTC LPLGVLMKGQ HLHLETFKAV LDGLDVLLAQ EVRPRRWKLQ VLDLRKNSHQ DFWTVWSGNR ASLYSFPEPE AAQPMTKKRK VDGLSTEAEQ PFIPVEVLVD LFLKEGACDE LFSYLIEKVK RKKNVLRLCC KKLKIFAMPM QDIKMILKMV QLDSIEDLEV TCTWKLPTLA KFSPYLGQMI NLRRLLLSHI HASSYISPEK EEQYIAQFTS QFLSLQCLQA LYVDSLFFLR GRLDQLLRHV MNPLETLSIT NCRLSEGDVM HLSQSPSVSQ LSVLSLSGVM LTDVSPEPLQ ALLERASATL QDLVFDECGI TDDQLLALLP SLSHCSQLTT LSFYGNSISI SALQSLLQHL IGLSNLTHVL YPVPLESYED IHGTLHLERL AYLHARLREL LCELGRPSMV WLSANPCPHC GDRTFYDPEP ILCPCFMPN
- Characteristics
- Human PRAME full-length ORF ( AAH39731, 1 a.a. - 509 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Preferentially Expressed Antigen in Melanoma (PRAME) (AA 321-420) protein (His-IF2DI Tag)
PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509) protein (His tag)Crystallography grade PRAME Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Preferentially Expressed Antigen in Melanoma (PRAME) (AA 1-509), (full length) protein (His tag)PRAME Origin: Human Host: Yeast Recombinant > 90 % SDS
Preferentially Expressed Antigen in Melanoma (PRAME) (Transcript Variant 4) protein (Myc-DYKDDDDK Tag)PRAME Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
-
- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
-
-
: "Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation." in: Cancer immunology, immunotherapy : CII, Vol. 63, Issue 11, pp. 1151-62, (2014) (PubMed).
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: "Functional autoantibodies against SSX-2 and NY-ESO-1 in multiple myeloma patients after allogeneic stem cell transplantation." in: Cancer immunology, immunotherapy : CII, Vol. 63, Issue 11, pp. 1151-62, (2014) (PubMed).
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- Target
- PRAME (Preferentially Expressed Antigen in Melanoma (PRAME))
- Alternative Name
- PRAME (PRAME Products)
- Background
-
Full Gene Name: preferentially expressed antigen in melanoma
Synonyms: MAPE,OIP4 - Gene ID
- 23532
- Pathways
- Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
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