PYCARD Protein (AA 1-149) (GST tag)
(1 reference)-
- Target See all PYCARD Proteins
- PYCARD (PYD and CARD Domain Containing (PYCARD))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-149
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This PYCARD protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- PYCARD (Human) Recombinant Protein (P01)
- Sequence
- MDALDLTDKL VSFYLETYGA ELTANVLRDM GLQEMAGQLQ AATHQGSGAA PAGIQAPPQS AAKPGLHFID QHRAALIARV TNVEWLLDAL YGKVLTDEQY QAVRAEPTNP SKMRKLFNFT PAWNWTCKDL LLQALRESQS YLVEDLERS
- Characteristics
- Human PYCARD full-length ORF ( AAH13569.2, 1 a.a. - 149 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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PYD and CARD Domain Containing (PYCARD) (AA 1-195) protein (His tag)
Crystallography grade PYCARD Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
PYD and CARD Domain Containing (PYCARD) (AA 1-193) protein (His tag)Crystallography grade PYCARD Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
PYD and CARD Domain Containing (PYCARD) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PYCARD Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).
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: "NLRP3 activation and mitosis are mutually exclusive events coordinated by NEK7, a new inflammasome component." in: Nature immunology, Vol. 17, Issue 3, pp. 250-8, (2016) (PubMed).
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- Target
- PYCARD (PYD and CARD Domain Containing (PYCARD))
- Alternative Name
- PYCARD (PYCARD Products)
- Synonyms
- ASC Protein, CARD5 Protein, TMS Protein, TMS-1 Protein, TMS1 Protein, 9130417A21Rik Protein, Asc Protein, TNS1 Protein, masc Protein, asc1 Protein, zAsc Protein, zgc:109874 Protein, PYD and CARD domain containing Protein, PYCARD Protein, Pycard Protein, pycard Protein
- Background
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Full Gene Name: PYD and CARD domain containing
Synonyms: ASC,CARD5,MGC10332,TMS,TMS-1,TMS1 - Gene ID
- 29108
- Pathways
- Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Actin Filament Polymerization, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation, Inflammasome
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