SMURF1 Protein (AA 1-731) (GST tag)
(1 reference)-
- Target See all SMURF1 Proteins
- SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-731
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This SMURF1 protein is labelled with GST tag.
- Application
- ELISA, Western Blotting (WB), Affinity Purification (AP), Antibody Array (AA)
- Purpose
- SMURF1 (Human) Recombinant Protein (P01)
- Sequence
- MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS TDTVKNTLDP KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL LSNAISRLKD TGYQRLDLCK LNPSDTDAVR GQIVVSLQTR DRIGTGGSVV DCRGLLENEG TVYEDSGPGR PLSCFMEEPA PYTDSTGAAA GGGNCRFVES PSQDQRLQAQ RLRNPDVRGS LQTPQNRPHG HQSPELPEGY EQRTTVQGQV YFLHTQTGVS TWHDPRIPRD LNSVNCDELG PLPPGWEVRS TVSGRIYFVD HNNRTTQFTD PRLHHIMNHQ CQLKEPSQPL PLPSEGSLED EELPAQRYER DLVQKLKVLR HELSLQQPQA GHCRIEVSRE EIFEESYRQI MKMRPKDLKK RLMVKFRGEE GLDYGGVARE WLYLLCHEML NPYYGLFQYS TDNIYMLQIN PDSSINPDHL SYFHFVGRIM GLAVFHGHYI NGGFTVPFYK QLLGKPIQLS DLESVDPELH KSLVWILEND ITPVLDHTFC VEHNAFGRIL QHELKPNGRN VPVTEENKKE YVRLYVNWRF MRGIEAQFLA LQKGFNELIP QHLLKPFDQK ELELIIGGLD KIDLNDWKSN TRLKHCVADS NIVRWFWQAV ETFDEERRAR LLQFVTGSTR VPLQGFKALQ GSTGAAGPRL FTIHLIDANT DNLPKAHTCF NRIDIPPYES YEKLYEKLLT AVEETCGFAV E
- Characteristics
- Human SMURF1 full-length ORF ( NP_851994.1, 1 a.a. - 731 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 165-268) protein (GST tag)
SMURF1 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 1-757) protein (His tag)Crystallography grade SMURF1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (AA 1-731) protein (His tag)Crystallography grade SMURF1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SMURF1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in ..." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 33667-81, (2013) (PubMed).
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: "Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad ubiquitination regulatory factor 1 (Smurf1) promote tribbles homolog 2 (TRIB2) stability and carcinogenic property in ..." in: The Journal of biological chemistry, Vol. 288, Issue 47, pp. 33667-81, (2013) (PubMed).
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- Target
- SMURF1 (SMAD Specific E3 Ubiquitin Protein Ligase 1 (SMURF1))
- Alternative Name
- SMURF1 (SMURF1 Products)
- Background
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Full Gene Name: SMAD specific E3 ubiquitin protein ligase 1
Synonyms: KIAA1625 - Gene ID
- 57154
- NCBI Accession
- NM_181349
- Pathways
- Regulation of Cell Size
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