YAP1 Protein (AA 1-504) (GST tag)
(1 reference)-
- Target See all YAP1 Proteins
- YAP1 (Yes-Associated Protein 1 (YAP1))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-504
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This YAP1 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- YAP1 (Human) Recombinant Protein (P01)
- Sequence
- MDPGQQPPPQ PAPQGQGQPP SQPPQGQGPP SGPGQPAPAA TQAAPQAPPA GHQIVHVRGD SETDLEALFN AVMNPKTANV PQTVPMRLRK LPDSFFKPPE PKSHSRQAST DAGTAGALTP QHVRAHSSPA SLQLGAVSPG TLTPTGVVSG PAATPTAQHL RQSSFEIPDD VPLPAGWEMA KTSSGQRYFL NHIDQTTTWQ DPRKAMLSQM NVTAPTSPPV QQNMMNSASG PLPDGWEQAM TQDGEIYYIN HKNKTTSWLD PRLDPRFAMN QRISQSAPVK QPPPLAPQSP QGGVMGGSNS NQQQQMRLQQ LQMEKERLRL KQQELLRQAM RNINPSTANS PKCQELALRS QLPTLEQDGG TQNPVSSPGM SQELRTMTTN SSDPFLNSGT YHSRDESTDS GLSMSSYSVP RTPDDFLNSV DEMDTGDTIN QSTLPSQQNR FPDYLEAIPG TNVDLGTLEG DGMNIEGEEL MPSLQEALSS DILNDMESVL AATKLDKESF LTWL
- Characteristics
- Human YAP1 full-length ORF ( AAH38235.1, 1 a.a. - 504 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Yes-Associated Protein 1 (YAP1) (AA 350-504) protein (His tag)
YAP1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Yes-Associated Protein 1 (YAP1) (AA 53-161) protein (GST tag)YAP1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Yes-Associated Protein 1 (YAP1) (AA 1-504) protein (Strep Tag)Crystallography grade YAP1 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Yes-Associated Protein 1 (YAP1) (AA 1-504), (full length) protein (His tag)YAP1 Origin: Human Host: Yeast Recombinant > 90 % SDS
Yes-Associated Protein 1 (YAP1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)YAP1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC)." in: PLoS ONE, Vol. 9, Issue 3, pp. e90836, (2014) (PubMed).
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: "Nicotine activates YAP1 through nAChRs mediated signaling in esophageal squamous cell cancer (ESCC)." in: PLoS ONE, Vol. 9, Issue 3, pp. e90836, (2014) (PubMed).
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- Target
- YAP1 (Yes-Associated Protein 1 (YAP1))
- Alternative Name
- YAP1 (YAP1 Products)
- Synonyms
- YAP Protein, YAP2 Protein, YAP65 Protein, YKI Protein, xyap Protein, yap Protein, yap2 Protein, yap65 Protein, yki Protein, AI325207 Protein, Yap Protein, Yap65 Protein, Yki Protein, Yorkie Protein, cb194 Protein, sb:cb194 Protein, si:ch211-181p1.5 Protein, si:dkey-3b8.3 Protein, wu:fc18c04 Protein, zgc:158380 Protein, Yes associated protein 1 Protein, Yes associated protein 1 S homeolog Protein, yes-associated protein 1 Protein, Yes-associated protein 1 Protein, YAP1 Protein, yap1.S Protein, Yap1 Protein, yap1 Protein
- Background
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Full Gene Name: Yes-associated protein 1, 65 kDa
Synonyms: YAP,YAP2,YAP65,YKI - Gene ID
- 10413
- Pathways
- MAPK Signaling, Stem Cell Maintenance, Regulation of Lipid Metabolism by PPARalpha
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