NAMPT Protein (AA 1-491) (His tag)

Catalog No. ABIN1344090

This Recombinant NAMPT protein is expressed in Escherichia coli (E. coli) and has been mentioned in 1 publication.

Quick Overview for NAMPT Protein (AA 1-491) (His tag) (ABIN1344090)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-491

Purification tag / Conjugate: This NAMPT protein is labelled with His tag.

Purpose: Nampt (Visfatin/PBEF) (mouse) (rec.) (His)

Cross-Reactivity: Mouse

Characteristics: Mouse Nampt (visfatin/PBEF) (aa 1-491) is fused at the C-terminus to a His-tag.

Sterility: Sterile filtered

Endotoxin Level: <1EU/μg purified protein (LAL test).

Biological Activity Comment: Shows adipogenic effects in stimulated differentiating 3T3-L1 cells.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in distilled water.

Buffer: Lyophilized from 0.2μm-filtered solution in PBS, pH 7.2.

Handling Advice: After reconstitution, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Brown, Onyango, Ramanjaneya, Conner, Patel, Dunmore, Randeva: "Visfatin regulates insulin secretion, insulin receptor signalling and mRNA expression of diabetes-related genes in mouse pancreatic beta-cells." in: Journal of molecular endocrinology, Vol. 44, Issue 3, pp. 171-8, (2010) (PubMed).

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt

Background:

Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase

Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an adipokine that is localized to the bloodstream and has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.

Molecular Weight: ~52kDa (SDS-PAGE)

UniProt: Q99KQ4