NAMPT Protein (AA 1-491) (DYKDDDDK Tag)

Catalog No. ABIN1344283

Recombinant NAMPT protein expressed in HEK-293 Cells and has been mentioned in 1 publication.

Quick Overview for NAMPT Protein (AA 1-491) (DYKDDDDK Tag) (ABIN1344283)

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Protein Type: Recombinant

Origin: Rat

Source: HEK-293 Cells

Application: SDS-PAGE (SDS)

Purity: >90 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 1-491

Purification tag / Conjugate: This NAMPT protein is labelled with DYKDDDDK Tag.

Purpose: Nampt (Visfatin/PBEF) (rat) (rec.)

Cross-Reactivity: Rat

Characteristics: Rat Nampt (visfatin/PBEF) (aa 1-491) is fused at the N-terminus to a FLAG®-tag.

Sterility: Sterile filtered

Endotoxin Level: <0.1EU/μg purified protein (LAL test).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.35 mg/mL

Buffer: 0.2μm-filtered solution in PBS, pH 7.2.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. For maximum product recovery after thawing, centrifuge the vial before opening the cap.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 6 months

References

Spinnler, Gorski, Stolz, Schuster, Garten, Beck-Sickinger, Engelse, de Koning, Körner, Kiess, Maedler: "The adipocytokine Nampt and its product NMN have no effect on beta-cell survival but potentiate glucose stimulated insulin secretion." in: PLoS ONE, Vol. 8, Issue 1, pp. e54106, (2013) (PubMed).

Target Details

Target: NAMPT (Nicotinamide phosphoribosyltransferase (NAMPT))

Alternative Name: Nampt

Background:

Pre-B Cell Colony Enhancing Factor 1, PBEF1, Nicotinamide Phosphoribosyltransferase

Nicotinamide phosphoribosyltransferase (Nampt, pre-B cell colony-enhancing factor, PBEF, Visfatin) is an 52 kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity. Recently NAMPT extracellular (eNAMPT) has been shown to be secreted from macrophages in muscles and to triggers a proliferative signal of muscle stem cell (MusC) by binding to CCR5 located at the surface receptor of MusC.

Molecular Weight: ~60kDa (SDS-PAGE)

UniProt: Q80Z29