CD40 Ligand Protein (CD40LG) (AA 18-111) (DYKDDDDK Tag)

Catalog No. ABIN1344285

Product Details

Target: CD40 Ligand (CD40LG)

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 18-111

Origin: Human, Mouse

Source: CHO Cells

Purification tag / Conjugate: This CD40 Ligand protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Brand: MegaCD40L™

Specificity: Binds to human and mouse CD40.

Cross-Reactivity: Human, Mouse (Murine)

Characteristics: Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.

Purity: >95 % (SDS-PAGE)

Endotoxin Level: <0.01EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized. Contains PBS.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C.

Expiry Date: 6 months

References

Guarda, Dostert, Staehli, Cabalzar, Castillo, Tardivel, Schneider, Tschopp: "T cells dampen innate immune responses through inhibition of NLRP1 and NLRP3 inflammasomes." in: Nature, Vol. 460, Issue 7252, pp. 269-73, (2009) (PubMed).

Holler, Tardivel, Kovacsovics-Bankowski, Hertig, Gaide, Martinon, Tinel, Deperthes, Calderara, Schulthess, Engel, Schneider, Tschopp: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).

Target Details

Target: CD40 Ligand (CD40LG)

Alternative Name: MegaCD40L (CD40LG Products)

Synonyms: CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein

Background: MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.

Molecular Weight: ~35-40kDa

UniProt: P27548

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints