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CD40 Ligand Protein (CD40LG) (AA 18-111) (DYKDDDDK Tag)

CD40LG Origin: Human Host: CHO Cells Recombinant >95 % (SDS-PAGE) SDS Active
Catalog No. ABIN1344289
  • Target See all CD40 Ligand (CD40LG) Proteins
    CD40 Ligand (CD40LG)
    Protein Type
    Recombinant
    Biological Activity
    Active
    Protein Characteristics
    AA 18-111
    Origin
    • 41
    • 22
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 36
    • 19
    • 10
    • 6
    • 5
    • 3
    • 3
    • 1
    • 1
    CHO Cells
    Purification tag / Conjugate
    This CD40 Ligand protein is labelled with DYKDDDDK Tag.
    Application
    SDS-PAGE (SDS)
    Brand
    MegaCD40L™
    Specificity
    Binds to human CD40.
    Cross-Reactivity
    Human
    Characteristics
    Human CD40L (aa 116-261) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
    Purity
    >95 % (SDS-PAGE)
    Endotoxin Level
    <0.01EU/μg purified protein (LAL test, Lonza).
    Top Product
    Discover our top product CD40LG Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86) (ED50: <1ng/ml).

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Concentration
    Lot specific
    Buffer
    Lyophilized. Contains PBS
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 6 months after receipt when stored at -20°C.
    Expiry Date
    6 months
  • Kaltenmeier, Gawanbacht, Beyer, Lindner, Trzaska, van der Merwe, Härter, Grüner, Fabricius, Lotfi, Schwarz, Schütz, Hönig, Schulz, Kern, Bommer, Schrezenmeier, Kirchhoff, Jahrsdörfer: "CD4+ T cell-derived IL-21 and deprivation of CD40 signaling favor the in vivo development of granzyme B-expressing regulatory B cells in HIV patients." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3768-77, (2015) (PubMed).

    French, Huard, Wysocka, Shane, Contassot, Arrighi, Piguet, Calderara, Rook: "Impaired CD40L signaling is a cause of defective IL-12 and TNF-alpha production in Sézary syndrome: circumvention by hexameric soluble CD40L." in: Blood, Vol. 105, Issue 1, pp. 219-25, (2004) (PubMed).

    Holler, Tardivel, Kovacsovics-Bankowski, Hertig, Gaide, Martinon, Tinel, Deperthes, Calderara, Schulthess, Engel, Schneider, Tschopp: "Two adjacent trimeric Fas ligands are required for Fas signaling and formation of a death-inducing signaling complex." in: Molecular and cellular biology, Vol. 23, Issue 4, pp. 1428-40, (2003) (PubMed).

  • Target
    CD40 Ligand (CD40LG)
    Alternative Name
    MegaCD40L (CD40LG Products)
    Synonyms
    CD154 Protein, cd40l Protein, CD40L Protein, HIGM1 Protein, IGM Protein, IMD3 Protein, T-BAM Protein, TNFSF5 Protein, TRAP Protein, gp39 Protein, hCD40L Protein, CD40-L Protein, Cd40l Protein, Ly-62 Protein, Ly62 Protein, Tnfsf5 Protein, CD40 ligand Protein, TNF superfamily member 5 Protein, CD40LG Protein, cd40l Protein, Cd40lg Protein
    Background
    MultimericCD40L™ is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to combinations. MultimericCD40L™ has shown to suppress alum-induced IL-1beta release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
    Molecular Weight
    ~35-40kDa (SDS-PAGE)
    UniProt
    P29965
    Pathways
    NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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