HES1 Protein (AA 2-280) (His tag)

Catalog No. ABIN1344335

Product Details

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Protein Type: Recombinant

Protein Characteristics: AA 2-280

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This HES1 protein is labelled with His tag.

Application: SDS-PAGE (SDS)

Cross-Reactivity: Human

Characteristics: Human HES1 (aa 2-280) is fused at the N-terminus to a His-tag.

Purity: >90 % (SDS-PAGE)

Sterility: 0.2 μm filtered

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in 55 mM TRIS-Cl, pH 8.2, containing 150 mM NaCl.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 3 months

Target Details

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1 (HES1 Products)

Synonyms: HES-1 Protein, HHL Protein, HRY Protein, bHLHb39 Protein, Hry Protein, HAIRY1 Protein, c-hairy1A Protein, Xhairy1 Protein, hairy1 Protein, hes1 Protein, Xhairy-1 Protein, Xlh1 Protein, hes family bHLH transcription factor 1 Protein, hairy and enhancer of split 1 (Drosophila) Protein, hes family bHLH transcription factor 4 Protein, hes family bHLH transcription factor 1 S homeolog Protein, hes family bHLH transcription factor 1 L homeolog Protein, HES1 Protein, Hes1 Protein, HES4 Protein, hes1.S Protein, hes1.L Protein

Background: BHLH proteins are essential for neurogenesis, myogenesis, hematopoiesis and sex determination. HES1 (Hairy and Enhancer of Split 1) is a basic Helix-Loop-Helix (bHLH) transcriptional repressor regulated by the Notch pathway and expressed in various tissues. HES1 is well known in development of different tissues, particularly endoderm derived endocrine organs like pancreas and neuroendocrine cells of lung. HES1 controls normal development by regulating proliferation and differentiation and so cell fate. Notch signaling plays both oncogenic and tumor suppressor roles depending on cell type. In contrast to T cell acute lymphoblastic leukemia (ALL), where Notch activation promotes leukemogenesis, induction of Notch signaling in B cell ALL (B-ALL) leads to growth arrest and apoptosis. The Notch target HES1 is sufficient to reproduce this tumor suppressor phenotype in B-ALL by interacting and activating PARP-1 and regulating proapoptotic signals.

Molecular Weight: ~35kDa (SDS-PAGE)

UniProt: Q14469

Pathways: DNA Damage Repair