TNFSF18 Protein (AA 45-173, Extracellular Domain, Soluble) (DYKDDDDK Tag)

Catalog No. ABIN1344404

Product Details

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: Soluble, Extracellular Domain, AA 45-173

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This TNFSF18 protein is labelled with DYKDDDDK Tag.

Application: SDS-PAGE (SDS)

Specificity: Binds to mouse GITR.

Cross-Reactivity: Mouse (Murine)

Characteristics: The extracellular domain of mouse GITRL (aa 45-173) is fused at the N-terminus to a FLAG®-tag.

Purity: >90 % (SDS-PAGE)

Sterility: 0.2 μm filtered

Endotoxin Level: <0.1EU/μg purified protein (LAL test, Lonza).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Induces GITR-mediated NF-kappaB activation in HEK 293 cells.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.2μm-filtered solution in 10 mM glycine, pH 10.0.

Storage: 4 °C,-20 °C

Storage Comment: Short Term Storage: +4°C
Long Term Storage: -20°C
Working aliquots are stable for up to 3 months when stored at -20°C.

Expiry Date: 3 months

Target Details

Target: TNFSF18 (Tumor Necrosis Factor (Ligand) Superfamily, Member 18 (TNFSF18))

Alternative Name: GITRL (TNFSF18 Products)

Synonyms: Gitrl Protein, AITRL Protein, GITRL Protein, TL6 Protein, hGITRL Protein, TNLG2A Protein, tumor necrosis factor (ligand) superfamily, member 18 Protein, TNF superfamily member 18 Protein, Tnfsf18 Protein, TNFSF18 Protein

Background: GITRL (Glucocorticoid-induced TNF receptor ligand) is expressed on dendritic cells (DC), monocytes, macrophages, B cells, activated T cells, endothelial cells, osteoclasts and various healthy non-lymphoid tissues (e.g. testis). GITRL is constitutively expressed and released as soluble form by solid tumors and various hematopoietic malignancies. GITRL causes differentiation of osteoclasts, activation of macrophages, but also alteration of carcinoma and leukemia cells and influences apoptosis. Binding to GITR is important in regulating T cell proliferation and TCR-mediated apoptosis. GITRL is implicated in development of autoimmune diseases and in the immune response against infectious pathogens and tumors.

Molecular Weight: ~22kDa (SDS-PAGE)

UniProt: Q7TS55