TNFSF4 Protein (AA 18-111, Extracellular Domain) (DYKDDDDK Tag)
(1 reference)-
- Target See all TNFSF4 Proteins
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 18-111, Extracellular Domain
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Origin
- Human, Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This TNFSF4 protein is labelled with DYKDDDDK Tag.
- Application
- SDS-PAGE (SDS)
- Brand
- MegaOX40L™
- Specificity
- Binds to human and mouse OX40 [CD134].
- Cross-Reactivity
- Human, Mouse (Murine)
- Characteristics
- The extracellular domain of mouse OX40L (aa 52-199) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag.
- Purity
- >90 % (SDS-PAGE)
- Endotoxin Level
- <0.01EU/μg purified protein (LAL test, Lonza).
- Top Product
- Discover our top product TNFSF4 Protein
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Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (Trimer) protein (His-DYKDDDDK Tag)
TNFSF4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Tris-Bis PAGE
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) protein (mFc-His Tag)TNFSF4 Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. FACS
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-183) (Active) protein (His tag)HPLC verified TNFSF4 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-198) protein (Fc Tag)TNFSF4 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 52-183) protein (His-DYKDDDDK-Strep II Tag)TNFSF4 Origin: Human Host: HEK-293T Cells Recombinant SDS, WB, ELISA, PI
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) protein (His tag)TNFSF4 Origin: Cynomolgus Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 1-183) protein (GST tag)TNFSF4 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-183) protein (His tag)Crystallography grade TNFSF4 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 51-198) protein (His tag)Crystallography grade TNFSF4 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4) (AA 1-183) protein (Strep Tag)Crystallography grade TNFSF4 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Activates T cell proliferation.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Concentration
- Lot specific
- Buffer
- Lyophilized. Contains PBS.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 6 months after receipt when stored at -20°C. - Expiry Date
- 6 months
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: "Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA." in: International immunology, Vol. 17, Issue 6, pp. 737-47, (2005) (PubMed).
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: "Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA." in: International immunology, Vol. 17, Issue 6, pp. 737-47, (2005) (PubMed).
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- Target
- TNFSF4 (Tumor Necrosis Factor (Ligand) Superfamily, Member 4 (TNFSF4))
- Alternative Name
- MegaOX40L (TNFSF4 Products)
- Synonyms
- TNFSF4 Protein, OX40L Protein, TNFRSF4 Protein, CD134L Protein, CD252 Protein, GP34 Protein, OX-40L Protein, OX4OL Protein, TXGP1 Protein, Ath-1 Protein, Ath1 Protein, Ox40l Protein, Txgp1l Protein, gp34 Protein, TNF superfamily member 4 Protein, tumor necrosis factor (ligand) superfamily, member 4 Protein, TNFSF4 Protein, Tnfsf4 Protein
- Background
- OX40L is a membrane-expressed cytokine that belongs to the TNF ligand family. It acts as costimulator through interaction with its ligand OX40 on T cells, stimulating T cell activation, proliferation and cytokine production. OX40L is expressed on antigen presenting cells including B cells, dendritic cells, mast cells and endothelium. MultimericOX40L is a high activity construct in which two trimeric OX40L ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively activates T cell proliferation.
- Molecular Weight
- ~40kDa (SDS-PAGE)
- UniProt
- P43488
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation, Cancer Immune Checkpoints
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