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Flagellin Protein (FliC)

FliC Origin: Human, Mouse Host: Salmonella typhimurium >95 % (SDS-PAGE) SDS Active
Catalog No. ABIN1344457
  • Target See all Flagellin (FliC) Proteins
    Flagellin (FliC)
    Biological Activity
    Active
    Origin
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse
    Source
    • 7
    • 5
    • 1
    Salmonella typhimurium
    Application
    SDS-PAGE (SDS)
    Specificity
    Binds to human and mouse TLR5 (Toll-like receptor 5).
    Cross-Reactivity
    Human, Mouse (Murine)
    Purity
    >95 % (SDS-PAGE)
    Endotoxin Level
    <0.01EU/μg purified protein (LAL test, Lonza).
    Top Product
    Discover our top product FliC Protein
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Activation of TLR5 in human epithelial cell assays based on NF-kappaB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, beta-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin (high Purity) at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1μg /mouse.

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute with 100 μL sterile water.
    Concentration
    Lot specific
    Buffer
    Lyophilized. Contains PBS.
    Storage
    4 °C,-20 °C
    Storage Comment
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 6 months after receipt when stored at -20°C.
    Expiry Date
    6 months
  • Target
    Flagellin (FliC)
    Alternative Name
    Flagellin (FliC Products)
    Synonyms
    hag Protein, flaG Protein, flagellin Protein, flagellin (flic) Protein, flagellin E Protein, flagellin protein flic Protein, fliC Protein, FliC Protein, fliC+F2048 Protein, CJJ81176_1339 Protein, C8J_1256 Protein
    Background
    Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
    UniProt
    P06179
    Pathways
    Inflammasome
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