AKR1B1 Protein (AA 1-316) (GST tag)

Catalog No. ABIN1344744

Product Details

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Protein Type: Recombinant

Protein Characteristics: AA 1-316

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This AKR1B1 protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: AKR1B1 (Human) Recombinant Protein (P01)

Sequence: MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF

Characteristics: Human AKR1B1 full-length ORF ( AAH00260.1, 1 a.a. - 316 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Atsriku, Hoffmann, Moghaddam, Kumar, Surapaneni: "In vitro metabolism of a novel JNK inhibitor tanzisertib: interspecies differences in oxido-reduction and characterization of enzymes involved in metabolism." in: Xenobiotica; the fate of foreign compounds in biological systems, Vol. 45, Issue 6, pp. 465-80, (2015) (PubMed).

Target Details

Target: AKR1B1 (Aldo-Keto Reductase Family 1, Member B1 (Aldose Reductase) (AKR1B1))

Alternative Name: AKR1B1 (AKR1B1 Products)

Synonyms: ADR Protein, ALDR1 Protein, ALR2 Protein, AR Protein, ALDRED Protein, ALR-P-I Protein, Akr1b3 Protein, Akr1b4 Protein, Aldr1 Protein, Alr Protein, RATALDRED Protein, akr1b7 Protein, zgc:86611 Protein, aldo-keto reductase family 1 member B Protein, aldo-keto reductase family 1, member B1 (aldose reductase) Protein, aldo-keto reductase family 1, member B1 (aldose reductase) S homeolog Protein, AKR1B1 Protein, Akr1b1 Protein, akr1b1.S Protein, akr1b1 Protein

Background: Full Gene Name: aldo-keto reductase family 1, member B1 (aldose reductase)
Synonyms: ADR,ALDR1,ALR2,AR,MGC1804

Gene ID: 231

Pathways: Metabolism of Steroid Hormones and Vitamin D, C21-Steroid Hormone Metabolic Process, Monocarboxylic Acid Catabolic Process