ARAF Protein (AA 1-606) (GST tag)
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- Target See all ARAF Proteins
- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-606
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This ARAF protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP), Kinase Activity Assay (KAA)
- Purpose
- ARAF (Human) Recombinant Protein (P01)
- Sequence
- MEPPRGPPAN GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS DGTPRGSPSP ASVSSGRKSP HSKSPAEQRE RKSLADDKKK VKNLGYRDSG YYWEVPPSEV QLLKRIGTGS FGTVFRGRWH GDVAVKVLKV SQPTAEQAQA FKNEMQVLRK TRHVNILLFM GFMTRPGFAI ITQWCEGSSL YHHLHVADTR FDMVQLIDVA RQTAQGMDYL HAKNIIHRDL KSNNIFLHEG LTVKIGDFGL ATVKTRWSGA QPLEQPSGSV LWMAAEVIRM QDPNPYSFQS DVYAYGVVLY ELMTGSLPYS HIGCRDQIIF MVGRGYLSPD LSKISSNCPK AMRRLLSDCL KFQREERPLF PQILATIELL QRSLPKIERS ASEPSLHRTQ ADELPACLLS AARLVP
- Characteristics
- Human ARAF full-length ORF ( AAH02466, 1 a.a. - 606 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-227) protein (His-IF2DI Tag)
ARAF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 151-250) protein (GST tag)ARAF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 221-320) protein (GST tag)ARAF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-609) protein (GST tag)ARAF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-606) protein (His tag)Crystallography grade ARAF Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) (AA 1-604) protein (His tag)Crystallography grade ARAF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF) protein (Myc-DYKDDDDK Tag)ARAF Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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- Target
- ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))
- Alternative Name
- ARAF (ARAF Products)
- Synonyms
- zgc:92074 Protein, wu:fk45h07 Protein, ARAF Protein, a-raf Protein, araf Protein, araf1 Protein, pks2 Protein, rafa1 Protein, A-RAF Protein, ARAF1 Protein, PKS2 Protein, RAFA1 Protein, 1200013E08Rik Protein, A-Raf Protein, AW495444 Protein, Araf1 Protein, A-Raf proto-oncogene, serine/threonine kinase Protein, A-Raf proto-oncogene, serine/threonine kinase L homeolog Protein, A-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Araf proto-oncogene, serine/threonine kinase Protein, araf Protein, ARAF Protein, araf.L Protein, araf.S Protein, Araf Protein
- Background
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Gene: v-raf murine sarcoma 3611 viral oncogene homolog
Synonyms: A-RAF, ARAF1, PKS2, RAFA1 - Gene ID
- 369
- Pathways
- MAPK Signaling, RTK Signaling, Hepatitis C
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