BDNF Protein (AA 1-255) (GST tag)
(1 reference)-
- Target See all BDNF Proteins
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-255
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This BDNF protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- BDNF (Human) Recombinant Protein (P02)
- Sequence
- MFHQVRRVMT ILFLTMVISY FGCMKAAPMK EANIRGQGGL AYPGVRTHGT LESVNGPKAG SRGLTSLADT FEHVIEELLD EDQKVRPNEE NNKDADLYTS RVMLSSQVPL EPPLLFLLEE YKNYLDAANM SMRVRRHSDP ARRGELSVCD SISEWVTAAD KKTAVDMSGG TVTVLEKVPV SKGQLKQYFY ETKCNPMGYT KEGCRGIDKR HWNSQCRTTQ SYVRALTMDS KKRIGWRFIR IDTSCVCTLT IKRGR
- Characteristics
- Human BDNF full-length ORF ( NP_733927.1, 1 a.a. - 255 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) protein (His-IF2DI Tag)
BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Brain-Derived Neurotrophic Factor (BDNF) (AA 1-247) protein (GST tag)BDNF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Brain-Derived Neurotrophic Factor (BDNF) (AA 129-247) protein (His tag)Crystallography grade BDNF Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Brain-Derived Neurotrophic Factor (BDNF) (AA 131-249) protein (His tag)Crystallography grade BDNF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Brain-Derived Neurotrophic Factor (BDNF) (AA 137-237), (partial) protein (GST tag)BDNF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % SDS, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Convection enhanced drug delivery of BDNF through a microcannula in a rodent model to strengthen connectivity of a peripheral motor nerve bridge model to bypass spinal cord injury." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 19, Issue 4, pp. 563-9, (2012) (PubMed).
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: "Convection enhanced drug delivery of BDNF through a microcannula in a rodent model to strengthen connectivity of a peripheral motor nerve bridge model to bypass spinal cord injury." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 19, Issue 4, pp. 563-9, (2012) (PubMed).
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- Target
- BDNF (Brain-Derived Neurotrophic Factor (BDNF))
- Alternative Name
- BDNF (BDNF Products)
- Synonyms
- ANON2 Protein, BULN2 Protein, bdnf-A Protein, BDNF Protein, bdnf Protein, brain derived neurotrophic factor Protein, brain-derived neurotrophic factor Protein, brain-derived neurotrophic factor L homeolog Protein, brain-derived neurotrophic factor S homeolog Protein, BDNF Protein, Bdnf Protein, bdnf Protein, bdnf.L Protein, bdnf.S Protein
- Background
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Full Gene Name: brain-derived neurotrophic factor
Synonyms: MGC34632 - Gene ID
- 627
- NCBI Accession
- NM_170731
- Pathways
- RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity
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