Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB) (AA 1-982) protein (GST tag)

Catalog No. ABIN1348172

Product Details

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Protein Type: Recombinant

Protein Characteristics: AA 1-982

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: GST tag

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: CBLB (Human) Recombinant Protein (P01)

Sequence: MANSMNGRNP GGRGGNPRKG RILGIIDAIQ DAVGPPKQAA ADRRTVEKTW KLMDKVVRLC QNPKLQLKNS PPYILDILPD TYQHLRLILS KYDDNQKLAQ LSENEYFKIY IDSLMKKSKR AIRLFKEGKE RMYEEQSQDR RNLTKLSLIF SHMLAEIKAI FPNGQFQGDN FRITKADAAE FWRKFFGDKT IVPWKVFRQC LHEVHQISSG LEAMALKSTI DLTCNDYISV FEFDIFTRLF QPWGSILRNW NFLAVTHPGY MAFLTYDEVK ARLQKYSTKP GSYIFRLSCT RLGQWAIGYV TGDGNILQTI PHNKPLFQAL IDGSREGFYL YPDGRSYNPD LTGLCEPTPH DHIKVTQEQY ELYCEMGSTF QLCKICAEND KDVKIEPCGH LMCTSCLTAW QESDGQGCPF CRCEIKGTEP IIVDPFDPRD EGSRCCSIID PFGMPMLDLD DDDDREESLM MNRLANVRKC TDRQNSPVTS PGSSPLAQRR KPQPDPLQIP HLSLPPVPPR LDLIQKGIVR SPCGSPTGSP KSSPCMVRKQ DKPLPAPPPP LRDPPPPPPE RPPPIPPDNR LSRHIHHVES VPSKDPPMPL EAWCPRDVFG TNQLVGCRLL GEGSPKPGIT ASSNVNGRHS RVGSDPVLMR KHRRHDLPLE GAKVFSNGHL GSEEYDVPPR LSPPPPVTTL LPSIKCTGPL ANSLSEKTRD PVEEDDDEYK IPSSHPVSLN SQPSHCHNVK PPVRSCDNGH CMLNGTHGPS SEKKSNIPDL SIYLKGDVFD SASDPVPLPP ARPPTRDNPK HGSSLNRTPS DYDLLIPPLG EDAFDALPPS LPPPPPPARH SLIEHSKPPG SSSRPSSGQD LFLLPSDPFV DLASGQVPLP PARRLPGENV KTNRTSQDYD QLPSCSDGSQ APARPPKPRP RRTAPEIHHR KPHGPEAALE NVDAKIAKLM GEGYAFEEVK RALEIAQNNV EVARSILREF AFPPPVSPRL NL

Characteristics: Human CBLB full-length ORF ( AAH32851.1, 1 a.a. - 982 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Gruber, Hinterleitner, Hermann-Kleiter, Meisel, Kleiter, Wang, Viola, Pfeifhofer-Obermair, Baier: "Cbl-b mediates TGF? sensitivity by downregulating inhibitory SMAD7 in primary T cells." in: Journal of molecular cell biology, Vol. 5, Issue 6, pp. 358-68, (2013) (PubMed).

Target Details

Target: Cbl Proto-Oncogene B, E3 Ubiquitin Protein Ligase (CBLB)

Alternative Name: CBLB (CBLB Products)

Synonyms: rnf56 Protein, cblb Protein, AI429560 Protein, AI851073 Protein, Cbl-b Protein, Cbl proto-oncogene B, E3 ubiquitin protein ligase S homeolog Protein, Cbl proto-oncogene B, E3 ubiquitin protein ligase L homeolog Protein, Casitas B-lineage lymphoma b Protein, Cbl proto-oncogene B Protein, cblb.S Protein, cblb.L Protein, Cblb Protein, CBLB Protein

Background: Full Gene Name: Cas-Br-M (murine) ecotropic retroviral transforming sequence b
Synonyms: DKFZp686J10223,DKFZp779A0729,DKFZp779F1443,FLJ36865,FLJ41152,Nbla00127,RNF56

Gene ID: 868