CDK2 Protein (AA 1-298) (GST tag)
(2 references)-
- Target See all CDK2 Proteins
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-298
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This CDK2 protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Antibody Array (AA), Affinity Purification (AP)
- Purpose
- CDK2 (Human) Recombinant Protein (P01)
- Sequence
- MENFQKVEKI GEGTYGVVYK ARNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS HRVLHRDLKP QNLLINTEGA IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL
- Characteristics
- Human CDK2 full-length ORF ( AAH03065, 1 a.a. - 298 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Cyclin-Dependent Kinase 2 (CDK2) (AA 1-298) (Active) protein (GST tag)
CDK2 Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-198) protein (His tag)CDK2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Cyclin-Dependent Kinase 2 (CDK2) (AA 211-298) protein (GST tag)CDK2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 2 (CDK2) (AA 1-346) protein (Strep Tag)Crystallography grade CDK2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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: "Peptide aptamers in label-free protein detection: 2. Chemical optimization and detection of distinct protein isoforms." in: Analytical chemistry, Vol. 81, Issue 9, pp. 3314-20, (2009) (PubMed).
: "Regulated activating Thr172 phosphorylation of cyclin-dependent kinase 4(CDK4): its relationship with cyclins and CDK "inhibitors"." in: Molecular and cellular biology, Vol. 26, Issue 13, pp. 5070-85, (2006) (PubMed).
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: "Peptide aptamers in label-free protein detection: 2. Chemical optimization and detection of distinct protein isoforms." in: Analytical chemistry, Vol. 81, Issue 9, pp. 3314-20, (2009) (PubMed).
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- Target
- CDK2 (Cyclin-Dependent Kinase 2 (CDK2))
- Alternative Name
- CDK2 (CDK2 Products)
- Synonyms
- p33(CDK2) Protein, A630093N05Rik Protein, wu:fa10c02 Protein, zgc:56598 Protein, zgc:77684 Protein, eg1 Protein, CDKN2 Protein, cdk2L Protein, cyclin dependent kinase 2 Protein, cyclin-dependent kinase 2 Protein, cyclin-dependent kinase 2 S homeolog Protein, CDK2 Protein, Cdk2 Protein, cdk2 Protein, cdk2.S Protein
- Background
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Full Gene Name: cyclin-dependent kinase 2
Synonyms: p33(CDK2) - Gene ID
- 1017
- Pathways
- PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA
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