CTCF Protein (AA 1-727) (GST tag)

Catalog No. ABIN1350629

Product Details

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Protein Type: Recombinant

Protein Characteristics: AA 1-727

Origin: Human

Source: Wheat germ

Purification tag / Conjugate: This CTCF protein is labelled with GST tag.

Application: ELISA, Western Blotting (WB), Antibody Array (AA), Affinity Purification (AP)

Purpose: CTCF (Human) Recombinant Protein (P01)

Sequence: MEGDAVEAIV EESETFIKGK ERKTYQRRRE GGQEEDACHL PQNQTDGGEV VQDVNSSVQM VMMEQLDPTL LQMKTEVMEG TVAPEAEAAV DDTQIITLQV VNMEEQPINI GELQLVQVPV PVTVPVATTS VEELQGAYEN EVSKEGLAES EPMICHTLPL PEGFQVVKVG ANGEVETLEQ GELPPQEDPS WQKDPDYQPP AKKTKKTKKS KLRYTEEGKD VDVSVYDFEE EQQEGLLSEV NAEKVVGNMK PPKPTKIKKK GVKKTFQCEL CSYTCPRRSN LDRHMKSHTD ERPHKCHLCG RAFRTVTLLR NHLNTHTGTR PHKCPDCDMA FVTSGELVRH RRYKHTHEKP FKCSMCDYAS VEVSKLKRHI RSHTGERPFQ CSLCSYASRD TYKLKRHMRT HSGEKPYECY ICHARFTQSG TMKMHILQKH TENVAKFHCP HCDTVIARKS DLGVHLRKQH SYIEQGKKCR YCDAVFHERY ALIQHQKSHK NEKRFKCDQC DYACRQERHM IMHKRTHTGE KPYACSHCDK TFRQKQLLDM HFKRYHDPNF VPAAFVCSKC GKTFTRRNTM ARHADNCAGP DGVEGENGGE TKKSKRGRKR KMRSKKEDSS DSENAEPDLD DNEDEEEPAV EIEPEPEPQP VTPAPPPAKK RRGRPPGRTN QPKQNQPTAI IQVEDQNTGA IENIIVEVKK EPDAEPAEGE EEEAQPAATD APNGDLTPEM ILSMMDR

Characteristics: Human CTCF full-length ORF ( AAH14267, 1 a.a. - 727 a.a.) recombinant protein with GST-tag at N-terminal.

Purification: in vitro wheat germ expression system

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue.

Restrictions: For Research Use only

Handling

Buffer: 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.

Handling Advice: Aliquot to avoid repeated freezing and thawing.

Storage: -80 °C

Storage Comment: Best use within three months from the date of receipt of this protein.

References

Al-Mahdawi, Sandi, Mouro Pinto, Pook: "Friedreich ataxia patient tissues exhibit increased 5-hydroxymethylcytosine modification and decreased CTCF binding at the FXN locus." in: PLoS ONE, Vol. 8, Issue 9, pp. e74956, (2013) (PubMed).

Shukla, Kavak, Gregory, Imashimizu, Shutinoski, Kashlev, Oberdoerffer, Sandberg, Oberdoerffer: "CTCF-promoted RNA polymerase II pausing links DNA methylation to splicing." in: Nature, Vol. 479, Issue 7371, pp. 74-9, (2011) (PubMed).

Li, Hu, Qiu, Ling, Chen, Wang, Hou, Vu, Hoffman: "CTCF regulates allelic expression of Igf2 by orchestrating a promoter-polycomb repressive complex 2 intrachromosomal loop." in: Molecular and cellular biology, Vol. 28, Issue 20, pp. 6473-82, (2008) (PubMed).

Target Details

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF (CTCF Products)

Synonyms: im:7152666 Protein, CTCF Protein, xctcf Protein, AW108038 Protein, CCCTC-binding factor Protein, CCCTC-binding factor (zinc finger protein) Protein, CTCF Protein, ctcf Protein, Ctcf Protein

Background: Full Gene Name: CCCTC-binding factor (zinc finger protein)
Synonyms:

Gene ID: 10664