BCKDHB Protein (AA 51-392) (His tag)

Catalog No. ABIN1459678

Product Details

Target: BCKDHB (Branched Chain Keto Acid Dehydrogenase E1, beta Polypeptide (BCKDHB))

Protein Type: Recombinant

Protein Characteristics: AA 51-392

Origin: Cow

Source: Yeast

Purification tag / Conjugate: This BCKDHB protein is labelled with His tag.

Application: ELISA

Sequence: VAHFTFQPDP EPVEYGQTQK MNLFQAVTSA LDNSLAKDPT AVIFGEDVAF GGVFRCTVGL RDKYGKDRVF NTPLCEQGIV GFGIGIAVTG ATAIAEIQFA DYIFPAFDQI VNEAAKYRYR SGDLFNCGSL TIRSPWGCVG HGALYHSQSP EAFFAHCPGI KVVVPRSPFQ AKGLLLSCIE DKNPCIFFEP KILYRAAVEQ VPVEPYNIPL SQAEVIQEGS DVTLVAWGTQ VHVIREVAAM AQEKLGVSCE VIDLRTILPW DVDTVCKSVI KTGRLLVSHE APLTGGFASE ISSTVQEECF LNLEAPISRV CGYDTPFPHI FEPFYIPDKW KCYDALRKMI NY

Specificity: Bos taurus (Bovine)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: BCKDHB (Branched Chain Keto Acid Dehydrogenase E1, beta Polypeptide (BCKDHB))

Alternative Name: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial (BCKDHB) (BCKDHB Products)

Synonyms: zgc:158777 Protein, BCKDHB Protein, E1B Protein, dJ279A18.1 Protein, branched chain keto acid dehydrogenase E1, beta polypeptide Protein, branched chain keto acid dehydrogenase E1 subunit beta Protein, branched chain keto acid dehydrogenase E1, beta polypeptide S homeolog Protein, branched chain ketoacid dehydrogenase E1, beta polypeptide Protein, BCKDHB Protein, bckdhb Protein, bckdhb.S Protein, Bckdhb Protein

Background: Recommended name: 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial.
EC= 1.2.4.4.
Alternative name(s): Branched-chain alpha-keto acid dehydrogenase E1 component beta chain.
Short name= BCKDE1B.
Short name= BCKDH E1-beta

UniProt: P21839