Perforin 1 Protein (AA 21-554) (His tag)

Catalog No. ABIN7478971

Product Details

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Protein Type: Recombinant

Protein Characteristics: AA 21-554

Origin: Mouse

Source: Yeast

Purification tag / Conjugate: This Perforin 1 protein is labelled with His tag.

Application: ELISA

Sequence: PCYTATRSEC KQKHKFVPGV WMAGEGMDVT TLRRSGSFPV NTQRFLRPDR TCTLCKNSLM RDATQRLPVA ITHWRPHSSH CQRNVAAAKV HSTEGVAREA AANINNDWRV GLDVNPRPEA NMRASVAGSH SKVANFAAEK TYQDQYNFNS DTVECRMYSF RLVQKPPLHL DFKKALRALP RNFNSSTEHA YHRLISSYGT HFITAVDLGG RISVLTALRT CQLTLNGLTA DEVGDCLNVE AQVSIGAQAS VSSEYKACEE KKKQHKMATS FHQTYRERHV EVLGGPLDST HDLLFGNQAT PEQFSTWTAS LPSNPGLVDY SLEPLHTLLE EQNPKREALR QAISHYIMSR ARWQNCSRPC RSGQHKSSHD SCQCECQDSK VTNQDCCPRQ RGLAHLVVSN FRAEHLWGDY TTATDAYLKV FFGGQEFRTG VVWNNNNPRW TDKMDFENVL LSTGGPLRVQ VWDADYGWDD DLLGSCDRSP HSGFHEVTCE LNHGRVKFSY HAKCLPHLTG GTCLEYAPQG LLGDPPGNRS GAVW

Specificity: Mus musculus (Mouse)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: Perforin 1 (PRF1) (Perforin 1 (Pore Forming Protein) (PRF1))

Alternative Name: Perforin-1 (Prf1) (PRF1 Products)

Synonyms: FLH2 Protein, HPLH2 Protein, P1 Protein, PFN1 Protein, PFP Protein, PRF1 Protein, Pfn Protein, Pfp Protein, Prf-1 Protein, Cyta Protein, RATCYTA Protein, LOC443187 Protein, perforin Protein, prf1 Protein, cytolysin Protein, perforin-1 Protein, perforin-1-like Protein, perforin 1 Protein, perforin 1 (pore forming protein) Protein, perforin Protein, perforin 1 L homeolog Protein, PRF1 Protein, Prf1 Protein, LOC443187 Protein, prf1 Protein, prf1.L Protein

Background: Recommended name: Perforin-1.
Short name= P1.
Alternative name(s): Cytolysin Lymphocyte pore-forming protein

UniProt: P10820

Pathways: Apoptosis, Caspase Cascade in Apoptosis