ARAF Protein (AA 1-604) (His tag)

Catalog No. ABIN1473934

Product Details

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Protein Type: Recombinant

Protein Characteristics: AA 1-604

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This ARAF protein is labelled with His tag.

Application: ELISA

Sequence: MEPPRGPPAS GAEPSRAVGT VKVYLPNKQR TVVTVRDGMS VYDSLDKALK VRGLNQDCCV VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RRQFYHSIQD LSGGSRQQEV PSNLSVNELL TPQGPSPFTQ QRDQEHFSFP APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS TDAAGRGGDG APRGSPSPAS VSSGRKSPHS KLPAEQRERK SLADEKKKVK NLGYRDSGYY WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK NEMQVLRKTR HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD MVQLIDVARQ TAQGMDYLHA KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD PNPYSFQSDV YAYGVVLYEL MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM RRLLTDCLKF QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA RLVP

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: Serine/threonine-protein kinase A-Raf (Araf) (ARAF Products)

Synonyms: zgc:92074 Protein, wu:fk45h07 Protein, ARAF Protein, a-raf Protein, araf Protein, araf1 Protein, pks2 Protein, rafa1 Protein, A-RAF Protein, ARAF1 Protein, PKS2 Protein, RAFA1 Protein, 1200013E08Rik Protein, A-Raf Protein, AW495444 Protein, Araf1 Protein, A-Raf proto-oncogene, serine/threonine kinase Protein, A-Raf proto-oncogene, serine/threonine kinase L homeolog Protein, A-Raf proto-oncogene, serine/threonine kinase S homeolog Protein, Araf proto-oncogene, serine/threonine kinase Protein, araf Protein, ARAF Protein, araf.L Protein, araf.S Protein, Araf Protein

Background: Recommended name: Serine/threonine-protein kinase A-Raf.
EC= 2.7.11.1.
Alternative name(s): Proto-oncogene A-Raf Proto-oncogene A-Raf-1

UniProt: P14056

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C