GCLC Protein (AA 1-637) (His tag)

Catalog No. ABIN1474163

Product Details

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Protein Type: Recombinant

Protein Characteristics: AA 1-637

Origin: Rat

Source: Yeast

Purification tag / Conjugate: This GCLC protein is labelled with His tag.

Application: ELISA

Sequence: MGLLSQGSPL SWEETQRHAD HVRRHGILQF LHIYHAVKDR HKDVLKWGDE VEYMLVSFDH ENRKVQLLLN GGDVLETLQE KGERTNPNHP TLWRPEYGSY MIEGTPGQPY GGTMSEFNTV EDNMRKRRKE ATSVLGEHQA LCTITSFPRL GCPGFTLPEH RPNPEEGGAS KSLFFPDEAI NKHPRFGTLT RNIRHRRGEK VVINVPIFKD KNTPSPFVET FPEDEEASKA SKPDHIYMDA MGFGMGNCCL QVTFQACSIS EARYLYDQLA TICPIVMALS AASPFYRGYV SDIDCRWGVI SASVDDRTRE ERGLEPLKNN RFKISKSRYD SIDSYLSKCG EKYNDIDLTI DTEIYEQLLE EGIDHLLAQH VAHLFIRDPL TLFEEKIHLD DANESDHFEN IQSTNWQTMR FKPPPPNSDI GWRVEFRPME VQLTDFENSA YVVFVVLLTR VILSYKLDFL IPLSKVDENM KVAQERDAVL QGMFYFRKDI CKGGNAVVDG CSKAQTSSEP SAEEYTLMSI DTIINGKEGV FPGLIPILNS YLENMEVDVD TRCSILNYLK LIKKRASGEL MTVARWMREF IANHPDYKQD SVITDEINYS LILKCNQIAN ELCECPELLG SGFRKAKYSG GKSDPSD

Specificity: Rattus norvegicus (Rat)

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Purity: > 90 %

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C, for extended storage, conserve at -20 °C or -80 °C.

Target Details

Target: GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))

Alternative Name: Glutamate--cysteine ligase catalytic subunit (Gclc) (GCLC Products)

Synonyms: GCLC Protein, gclc Protein, MGC146287 Protein, 2259 Protein, CG2259 Protein, DmGCLC Protein, DmGCS Protein, DmGCSh Protein, Dmel\\CG2259 Protein, GCL Protein, GCLc Protein, GCSh Protein, Gcs Protein, Gcsh Protein, DDBDRAFT_0186120 Protein, DDBDRAFT_0231403 Protein, DDB_0186120 Protein, DDB_0231403 Protein, GCS Protein, GLCL Protein, GLCLC Protein, D9Wsu168e Protein, GLCL-H Protein, Ggcs-hs Protein, Glclc Protein, cb1049 Protein, fe36e11 Protein, wu:fe36e11 Protein, glutamate-cysteine ligase catalytic subunit Protein, glutamate-cysteine ligase, catalytic subunit Protein, Glutamate-cysteine ligase catalytic subunit Protein, gamma glutamylcysteine synthetase Protein, glutamate-cysteine ligase, catalytic subunit L homeolog Protein, glutamate-cysteine ligase, catalytic subunit S homeolog Protein, gamma-glutamylcysteine synthetase Protein, GCLC Protein, gclc Protein, Gclc Protein, LbGCS Protein, gcsA Protein, gclc.L Protein, gclc.S Protein, GSH1 Protein

Background: Recommended name: Glutamate--cysteine ligase catalytic subunit.
EC= 6.3.2.2.
Alternative name(s): GCS heavy chain Gamma-.
ECS Gamma-glutamylcysteine synthetase

UniProt: P19468

Pathways: Cell RedoxHomeostasis