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Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (Active) Protein Protein

UBA1 Origin: Human Source: Escherichia coli (E. coli) Recombinant SDS-PAGE: ≥95 % Func, SDS, WB
Catalog No. ABIN1525877
$370.00
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50 μg
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  • Target
    UBA1
    Protein Type
    Recombinant
    Biological Activity
    Active
    Origin
    Human
    Source
    Escherichia coli (E. coli)
    • 2
    • 1
    • 1
    • 1
    Application
    Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
    Purpose
    >95% Pure Human recombinant Ubiquitin Activating Enzyme E1
    Purity
    SDS-PAGE: ≥95 %
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Biological Activity: Typical enzyme concentration of 5 nM - 100 nM is used for in vitro conjugation, depending on conditions.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    In 50 mM Tris ( pH 8.0), 100 mM NaCl and 10 % glycerol.
    Handling Advice
    Centrifuge the vial prior to opening.
    Storage
    -80 °C
    Storage Comment
    -80°C
    Expiry Date
    12 months
  • Target
    UBA1
    Synonyms
    A1S9, A1S9T, A1ST, AMCX1, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, DDBDRAFT_0190927, DDBDRAFT_0220497, DDB_0190927, DDB_0220497, Sbx, Ube-1, Ube1x, a1s9, a1s9t, a1st, amcx1, gxp1, poc20, smax2, uba1a, ube1, ube1x, uba1, uba1b, ube, zgc:66143, wu:fa01e08, wu:fb30f01, wu:fi21c11, wu:fj14g11, ubiquitin like modifier activating enzyme 1, ubiquitin activating enzyme E1, Ubiquitin-conjugating enzyme E1, ubiquitin-like modifier activating enzyme 1, ubiquitin-like modifier activating enzyme 1 S homeolog, ubiquitin-like modifier activating enzyme 1 L homeolog, UBA1, uae1, GL50803_4083, GL50803_10661, Uba1, uba1.S, uba1.L, uba1, ptr3
    Background
    UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.
    Alternate Names: UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2
    Molecular Weight
    118 kDa
    Gene ID
    7317
    UniProt
    P22314
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