Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) (Active) Protein

Details for Product No. ABIN1525877
Protein Name
Origin
Human
5
Source
Escherichia coli (E. coli)
2
1
1
1
Protein Type
Recombinant
Biological Activity
Active
Application
Functional Studies (Func), SDS-PAGE (SDS), Western Blotting (WB)
Options
Purpose >95% Pure Human recombinant Ubiquitin Activating Enzyme E1
Purity SDS-PAGE: ≥95 %
Protein Name
Background UBE1, the ubiquitin activating enzyme, is the first enzyme utilized in the pathway to conjugate ubiquitin onto target substrates through an ubiquitin-protein isopeptide bond. The C-terminal glycine of an ubiquitin (G76) is initially adenylated in an ATP dependent manner activating it so it can be transferred to the active site cysteine of UBE1 forming an ubiquitin-UBE1 thiolester. With the help of the other enzymes in the ubiquitin conjugation pathway (E2/E3 enzymes) the ubiquitin is transferred to a specific lysine on a target substrate.
Alternate Names: UBE1, UBA1, POC20, A1S9T, A1ST, AMCX1, GXP1, SMAX2
Molecular Weight 118 kDa
Gene ID 7317
UniProt P22314
Application Notes Optimal working dilution should be determined by the investigator.
Comment

Biological Activity: Typical enzyme concentration of 5 nM - 100 nM is used for in vitro conjugation, depending on conditions.

Restrictions For Research Use only
Format Liquid
Buffer In 50 mM Tris ( pH 8.0), 100 mM NaCl and 10 % glycerol.
Handling Advice Centrifuge the vial prior to opening.
Storage -80 °C
Storage Comment -80°C
Expiry Date 12 months