Chemokine (C-C Motif) Ligand 15 (CCL15) Protein

Details for Product No. ABIN1540309
Protein Name
Origin
Human
32
Source
Escherichia coli (E. coli)
21
4
2
2
Protein Type
Recombinant
Options
Sequence QFTNDAETEL MMSKLPLENP VVLNSFHFAA DCCTSYISQS IPCSLMKSYF ETSSECSKPG VIFLTKKGRQ VCAKPSGPGV QDCMKKLKPY SI
Specificity A single non-glycosylated polypeptide chain containing 92 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analysis
Sterility 0.2 μm filtered
Endotoxin Level Less than 1EU/mg of rHuMIP-5/CCL15 as determined by LAL method.
Protein Name
Background Human MIP-5/CCL15 CCL15, a new human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 amino acids that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1gamma, respectively. The gene for MIP-5 is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-5 is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.
Molecular Weight 10.1 kDa
Restrictions For Research Use only
Format Lyophilized
Reconstitution It is recommended that sterile phosphate-buffered saline containing 1mg aluminum hydroxide be added to the vial to prepare a stock solution.
Buffer Lyophilized from a 0.2 µm filtered concentrated (1.0mg/mL) solution in 20mM PB, pH 7.4, 100mM NaCl.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage 4 °C/-20 °C
Storage Comment This liquid suspension is stable for several months at 0-4°C, but should be kept at -20°C for long term storage. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C.